The influence of storage time on ponazuril concentrations in feline plasma

PeerJ

article

Sherry Cox¹  Lainey Harvill¹  Sarah Singleton¹  Joan B. Bergman¹  Becky DeBolt² 

[1] Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee;Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee

关键词: Ponazuril;    Stability;    Storage;    Plasma;   

DOI  :  10.7717/peerj.12516

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe pharmacokinetics of ponazuril have been determined in several species; however, there is very little information on the stability of the drug after storage for long periods of time. This study was undertaken to determine the stability of ponazuril in plasma samples stored at −80 °C, which is the temperature most commonly used in the author’s laboratory.MethodSpiked plasma samples (0.3, 7.5, and 15 µg/mL) were stored at −80 °C for three months. Analysis occurred on the first day and then once a week for the following twelve weeks. The drug was extracted using a chloroform extraction and separated by high performance liquid chromatography using ultraviolet detection.ResultsThere was no loss of drug for any concentration for the first four weeks of storage. There was an average loss of less than 5% from day 35 through day 70 and an average loss of 6% on day 77 and 84. The data suggest that ponazuril is stable for 4 weeks when stored at −80 °C and undergoes minimal loss in the remaining 8 weeks.

【 授权许可】

CC BY