Genome-wide analysis of the CalS gene family in cotton reveals their potential roles in fiber development and responses to stress

PeerJ

article

Jiajia Feng¹  Yi Chen¹  Xianghui Xiao²  Yunfang Qu¹  Pengtao Li²  Quanwei Lu¹  Jinling Huang¹ 

[1]College of Agriculture, Shanxi Agricultural University

[2]School of Biotechnology and Food Engineering, Anyang Institute of Technology

关键词: Callose synthase;    Synteny;    Gene family;    Gossypium;    Various stresses;   

DOI  :  10.7717/peerj.12557

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Callose deposition occurs during plant growth and development, as well as when plants are under biotic and abiotic stress. Callose synthase is a key enzyme for the synthesis of callose. In this study, 27, 28, 16, and 15 callose synthase family members were identified in Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum using the sequence of Arabidopsis callose synthase. The CalSs were divided into five groups by phylogenetic, gene structure, and conservative motif analysis. The conserved motifs and gene structures of CalSs in each group were highly similar. Based on the analysis of cis-acting elements, it is inferred that GhCalSs were regulated by abiotic stress. WGD/Segmental duplication promoted the amplification of the CalS gene in cotton, and purification selection had an important function in the CalS family. The transcriptome data and qRT-PCR under cold, heat, salt, and PEG treatments showed that GhCalSs were involved in abiotic stress. The expression patterns of GhCalSs were different in various tissues. We predicted that GhCalS4, which was highly expressed in fibers, had an important effect on fiber elongation. Hence, these results help us understand the role of GhCalSs in fiber development and stress response.

【 授权许可】

CC BY