Extraction behaviors of aqueous PEG impregnated resin system in terms of impregnation stability and recovery via protein impregnated resin interactions on bovine serum albumin

PeerJ

article

Nur Fazrin Husna Abdul Aziz¹  Sahar Abbasiliasi²  Mazni Abu Zarin¹  Hui Suan Ng⁴  Chiwei Lan⁵  Joo Shun Tan¹ 

[1] School of Industrial Technology, Universiti Sains Malaysia;Halal Products Research Institute, Universiti Putra Malaysia;Institute of Bioscience, Universiti Putra Malaysia;Faculty of Applied Sciences, UCSI University;Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University

关键词: Aqueous impregnated resin system;    Bovine serum albumin;    Impregnation stability;    Adsorption kinetics;    Polyethylene glycol;   

DOI  :  10.7717/peerj.11920

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundCurrent advances in biotechnology have been looked at as alternative approaches towards the limited product recovery due to time- and cost-consuming drawbacks on the conventional purification methods. This study aimed to purify bovine serum albumin (BSA) as an exemplary target product using an aqueous impregnated resin system (AIRS). This method implies the concept of hydrophobicity of polymer that impregnated into the resins and driven by electrostatic attractions and hydrophilicity of aqueous salt solution to extract the target product.MethodsThe extraction behaviors of impregnation in terms of stability and adsorption kinetics via protein-aqueous polymer impregnated resin were studied. Impregnation stability was determined by the leaching factor of polyethylene glycol (PEG). The major factors such as PEG molecular weights and concentration, pH of aqueous salt solution, extraction methods (sonication and agitation) and types of adsorbent material and concentration of aqueous salt phase influencing on partitioning of biomolecule were also investigated.ResultsFor impregnation stability, the leaching factor for Amberlite XAD4 did not exceed 1%. The scanning electron microscopy (SEM) image analysis of Amberlite XAD4 attributes the structural changes with impregnation of resins. For adsorption kinetics, Freundlich adsorption isotherm with the highest R2 value (0.95) gives an indication of favorable adsorption process. Performance of AIRS impregnated with 40% (w/w) of PEG 2000 was found better than aqueous-two phase system (ATPS) by yielding the highest recovery of BSA (53.72%). The outcomes of this study propound the scope for the application of AIRS in purification of biomolecules.

【 授权许可】

CC BY