Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques

PeerJ

article

Dina M. Metwally¹  Reem Alajmi²  Muslimah N. Alsulami³  Isra M. Al-Turaiki⁴  Rewaida Abdel-Gaber²  Afrah F. Alkhuriji²  Haleema H. Albohiri³  Khalil Mohamed⁶  Hanadi B. Baghdadi⁷  Manal F. El-Khadragy⁹  Guillermo T. Isaias^1,11  Saeed El-Ashram^1,11 

[1] Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University;Department of Zoology, Faculty of Science, King Saud University;Department of Biology, College of Science, University of Jeddah;Department of Information Technology, College of Computer and Information Sciences, King Saud University;Zoology Department, Faculty of Science, Cairo University;Epidemioligy Department, Faculty of Public Health and Health Informatics, Umm Al-Qura University;Biology Department, College of Science, Imam Abdulrahman Bin Faisal University;Basic and Applied Scientific Research Center, Imam Abdulrahman Bin Faisal University;Department of Biology, Faculty of Science, Princess Nourah Bint Abdulrahman University;Department of Zoology and Entomology, Faculty of Science, University of Helwan;Department of Poultry Science, University of Arkansas;Faculty of Science, Kafrelsheikh University;College of Life Science and Engineering, Foshan University, Foshan, Guangdong Province

关键词: Theileria spp.;    Polymerase chain reaction (PCR);    18S rRNA;   

DOI  :  10.7717/peerj.12596

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications.MethodsBlood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing.ResultsAccording to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4–99.8% pairwise identity and 52.35–99.79% coverage.

【 授权许可】

CC BY