Early detection of cyanide, organophosphate and rodenticide pollution based on locomotor activity of zebrafish larvae

PeerJ

article

Binjie Wang¹  Junhao Zhu¹  Anli Wang¹  Jiye Wang¹  Yuanzhao Wu¹  Weixuan Yao¹ 

[1] The Department of Criminal Science and Technology, Zhejiang Police College, Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Hangzhou, Zhejiang province;College of Biosystems Engineering and Food Science, Zhejiang University, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Hangzhou, Zhejiang Province

关键词: Aquatic contamination;    Rodenticide;    Danio rerio;    Monitoring system;    Early warning;   

DOI  :  10.7717/peerj.12703

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Cyanide, organophosphate and rodenticides are highly toxic substances widely used in agriculture and industry. These toxicants are neuro- and organotoxic to mammals at low concentrations, thus early detection of these chemicals in the aqueous environment is of utmost importance. Here, we employed the behavioral toxicity test with wildtype zebrafish larvae to determine sublethal concentrations of the above mentioned common environmental pollutants. After optimizing the test with cyanide, nine rodenticides and an organophosphate were successfully tested. The compounds dose-dependently initially (0–60-min exposure) stimulated locomotor activity of larvae but induced toxicity and reduced swimming during 60–120-min exposure. IC50 values calculated based on swimming distance after 2-h exposure, were between 0.1 and 10 mg/L for both first-generation and second-generation anticoagulant rodenticides. Three behavioral characteristics, including total distance travelled, sinuosity and burst count, were quantitatively analyzed and compared by hierarchical clustering of the effects measured by each three parameters. The toxicity results for all three behavioral endpoints were consistent, suggesting that the directly measured parameter of cumulative swimming distance could be used as a promising biomarker for the aquatic contamination. The optimized method herein showed the potential for utilization as part of a monitoring system and an ideal tool for the risk assessment of drinking water in the military and public safety.

【 授权许可】

CC BY