Identification of HMMR as a prognostic biomarker for patients with lung adenocarcinoma via integrated bioinformatics analysis

PeerJ

article

Zhaodong Li¹  Hongtian Fei²  Siyu Lei¹  Fengtong Hao¹  Lijie Yang¹  Wanze Li¹  Laney Zhang³  Rui Fei¹ 

[1] Department of Cell Biology, College of Basic Medical Sciences, Jilin University;Department of Pharmacology, College of Basic Medical Sciences, Jilin University;The College of Arts and Sciences, Cornell University;Key Laboratory of Lymphatic Surgery Jilin Province, Jilin University

关键词: Lung adenocarcinoma;    Prognosis;    HMMR;    Bioinformatics;    Differentially expressed genes;   

DOI  :  10.7717/peerj.12624

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Lung adenocarcinoma (LUAD) is the most prevalent tumor in lung carcinoma cases and threatens human life seriously worldwide. Here we attempt to identify a prognostic biomarker and potential therapeutic target for LUAD patients. Methods Differentially expressed genes (DEGs) shared by GSE18842, GSE75037, GSE101929 and GSE19188 profiles were determined and used for protein-protein interaction analysis, enrichment analysis and clinical correlation analysis to search for the core gene, whose expression was further validated in multiple databases and LUAD cells (A549 and PC-9) by quantitative real-time PCR (qRT-PCR) and western blot analyses. Its prognostic value was estimated using the Kaplan-Meier method, meta-analysis and Cox regression analysis based on the Cancer Genome Atlas (TCGA) dataset and co-expression analysis was conducted using the Oncomine database. Gene Set Enrichment Analysis (GSEA) was performed to illuminate the potential functions of the core gene. Results A total of 115 shared DEGs were found, of which 24 DEGs were identified as candidate hub genes with potential functions associated with cell cycle and FOXM1 transcription factor network. Among these candidates, HMMR was identified as the core gene, which was highly expressed in LUAD as verified by multiple datasets and cell samples. Besides, high HMMR expression was found to independently predict poor survival in patients with LUAD. Co-expression analysis showed that HMMR was closely related to FOXM1 and was mainly involved in cell cycle as suggested by GSEA. Conclusion HMMR might be served as an independent prognostic biomarker for LUAD patients, which needs further validation in subsequent studies.

【 授权许可】

CC BY