Macrophage immunomodulatory activity of Acanthopanax senticousus polysaccharide nanoemulsion via activation of P65/JNK/ikkαsignaling pathway and regulation of Th1/Th2 Cytokines

PeerJ

article

Xianghui Li¹  Zhiqiang Zhang³  Zhenhuan Guo²  Li Zhao²  Yonglu Liu²  Xia Ma²  Qigai He¹ 

[1] State Key Laboratory of Agricultural Microbiology/College of Veterinary Medicine, Huazhong Agricultural University;Medicinal Engineering Department of Henan University of Animal Husbandry and Economy;Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine;Research Center for the inheritance and innovation of Chinese veterinary medicine classic prescriptions, Henan University of Animal Husbandry and Economy

关键词: Nanoemulsion;    Acanthopanax senticosus polysaccharide;    Immune responses;    T lymphocyte;    Pseudo-ternary phase diagram;   

DOI  :  10.7717/peerj.12575

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Nanoemulsions (NE) are used widely in pharmaceutical drug formulations and vaccine preparation, and Acanthopanax senticousus polysaccharide (ASPS) is a natural bioactive compound with immunostimulatory activity. Therefore, NE-loaded ASPS is expected to provide immunological enhancement for effective treatment. In the present study, Acanthopanax senticousus polysaccharide (ASPS was encapsulated into nanoemulsions, the resultant ASPS–NE were coated with a negative charge, and the immune enhancement mechanism of these ASPS-NE formulations was analyzed. The immunosuppressive animal models (70 ICR mice, male) for the study were established using cyclophosphamide. In addition, the activation of splenocyte proliferation, phagocytosis of the macrophages, the ratio of CD4+ to CD8+, the concentrations of the cytokines in serum, Western blot analysis was used for the analysis of the P65/JNK/ikk α signaling pathway in the peritoneal macrophage s. The results revealed that the ASPS-NE could stimulated the proliferation of splenocytes and enhance immunity. The ASPS-NE induced the expression of different cytokines (TNF-α, IFN-γ, IL-2, and IL-6), could activate the expressions of P65, JNK, and ikkα, and regulated the Th1/Th2 cytokines. These findings demonstrated the potential of ASPS-NE formulations for drug delivery and to induce potent and sustained immune responses.

【 授权许可】

CC BY