Role of miR-100-5p and CDC25A in breast carcinoma cells

PeerJ

article

Xiaoping Li¹  Yanli Ren³  Donghong Liu⁴  Xi Yu¹  Keda Chen² 

[1] Faculty of Medicine, Macau University of Science and Technology;Shulan International Medical College, Zhejiang Shuren University, Hangzhou, Zhejiang province;State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang province;Department of Laboratory Medicine, Hangyan hospital of Wenzhou Medical University, Taizhou First People’s Hospital

关键词: MiR-100-5p;    CDC25A;    Breast carcinoma;    Proliferation;    Migration and invasion;   

DOI  :  10.7717/peerj.12263

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

ObjectiveTo inquiry about mechanism of miR-100-5p/CDC25A axis in breast carcinoma (BC), thus offering a new direction for BC targeted treatment.MethodsqRT-PCR was employed to explore miR-100-5p and CDC25A mRNA levels. Western blot was employed for detecting protein expression of CDC25A. Targeting relationship of miR-100-5p and CDC25A was verified by dual-luciferase assay. In vitro experiments were used for assessment of cell functions.ResultsIn BC tissue and cells, miR-100-5p was significantly lowly expressed (P < 0.05) while CDC25A was highly expressed. Besides, miR-100-5p downregulated CDC25A level. miR-100-5p had a marked influence on the prognosis of patients. The forced miR-100-5p expression hindered BC cell proliferation, migration and invasion, and facilitated cell apoptosis. Upregulated miR-100-5p weakened promotion of CDC25A on BC cell growth.ConclusionTogether, these findings unveiled that CDC25A may be a key target of miR-100-5p that mediated progression of BC cells. Hence, miR-100-5p overexpression or CDC25A suppression may contribute to BC diagnosis.

【 授权许可】

CC BY