RNA-seq and phytohormone analysis reveals the culm color variation of Bambusa oldhamii Munro

PeerJ

article

Yulian Jiao¹  Hu Zeng³  Haitao Xia¹  Yueying Wang¹  Jinwang Wang¹  Chuan Jin¹ 

[1] Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences;Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-efficiency Utilization;Wuhan Metware Biotechnology Co., Ltd

关键词: Bambusa oldhamii;    Culm color variation;    Phytohormone;    Transcription factors;    MYB;    HY5;    ABA;    GA1;    GA7;    RNA-seq;   

DOI  :  10.7717/peerj.12796

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe clumping bamboo Bambusa oldhamii Munro, known as “green bamboo”, is famous for its edible bamboo shoots and fast-growing timber. The green and yellow striped-culm B. oldhamii variety, named B. oldhamii f. revoluta W.T. Lin & J. Y. Lin, is an attractive system for researching the culm color variation of B. oldhamii.MethodsMillions of clean reads were generated and assembled into 604,900 transcripts, and 383,278 unigenes were acquired with RNA-seq technology. The quantification of ABA, IAA, JA, GA1, GA3, GA4, and GA7 was performed using HPLC–MS/MS platforms.ResultsDifferential expression analysis showed that 449 unigenes were differentially expressed genes (DEGs), among which 190 DEGs were downregulated and 259 DEGs were upregulated in B. oldhamii f. revoluta. Phytohormone contents, especially GA1 and GA7, were higher in B. oldhamii. Approximately 21 transcription factors (TFs) were differentially expressed between the two groups: the bZIP, MYB, and NF-YA transcription factor families had the most DEGs, indicating that those TFs play important roles in B. oldhamii culm color variation. RNA-seq data were confirmed by quantitative RT-PCR analysis of the selected genes; moreover, phytohormone contents, especially those of ABA, GA1 and GA7, were differentially accumulated between the groups. Our study provides a basal gene expression and phytohormone analysis of B. oldhamii culm color variation, which could provide a solid fundamental theory for investigating bamboo culm color variation.

【 授权许可】

CC BY