AgGMP encoding GDP-D-mannose pyrophosphorylase from celery enhanced the accumulation of ascorbic acid and resistance to drought stress in Arabidopsis

PeerJ

article

Yan-Hua Liu¹  Hao Wang¹  Jie-Xia Liu¹  Sheng Shu¹  Guo-Fei Tan³  Meng-Yao Li⁴  Ao-Qi Duan¹  Hui Liu¹  Ai-Sheng Xiong¹ 

[1] State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University;Suqian Academy of Protected Horticultures;Institute of Horticulture, Guizhou Academy of Agricultural Sciences;College of Horticulture, Sichuan Agricultural University

关键词: Apium graveolens;    Arabidopsis thaliana;    Ascorbic acid;    GDP-D-mannose pyrophosphorylase;    Drought stress;    Gene expression;   

DOI  :  10.7717/peerj.12976

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Ascorbic acid (AsA) is an important nutrient in celery, the conversion of D-mannose-1-P to GDP-D-mannose catalyzed by GDP-D-mannose pyrophosphorylase (GMPase) represents the first committed step in the biosynthesis of AsA. To clarify the function of the AgGMP gene of celery, the AgGMP gene was cloned from celery cv. ‘Jinnan Shiqin’ . It contains an open reading frame (ORF) with the length of 1,086 bp, encoding 361 amino acids. AgGMP protein was highly conserved among different plant species. Phylogenetic analysis demonstrated that the GMP proteins from celery and carrot belonged to the same branch. AgGMP protein was mainly composed of three α-helixes and certain random coils. No signal peptide was found in the AgGMP protein. The subcellular localization indicated that the AgGMP protein was located in the cytoplasm. The relative expression levels of AgGMP in ‘Jinnan Shiqin’ were significantly up-regulated at 2 h and 4 h under drought stress treatments. AsA contents in transgenic Arabidopsis lines hosting AgGMP gene were higher than that in wild type plants, and the root lengths were also longer in the MS medium containing 300 mM mannitol. The present study provides useful evidence for the functional involvement of AgGMP in regulating AsA accumulation and response to drought stress in celery.

【 授权许可】

CC BY