Genome wide expression analysis of circular RNAs in mammary epithelial cells of cattle revealed difference in milk synthesis

PeerJ

article

Syed Mudasir Ahmad¹  Basharat Bhat¹  Zainab Manzoor²  Mashooq Ahmad Dar¹  Qamar Taban¹  Eveline M. Ibeagha-Awemu³  Nadeem Shabir¹  Mohd Isfaqul Hussain⁴  Riaz A. Shah¹  Nazir A. Ganai¹ 

[1] Division of Animal Biotechnology, Faculty of Veterinary Sciences, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir;Department of Clinical Biochemistry, University of Kashmir;Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada;Division of Veterinary Microbiology

关键词: CircRNA;    Mammary gland;    Bovine;    RNA-Seq;    Identification;    Differential expression;   

DOI  :  10.7717/peerj.13029

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Milk is an excellent source of nutrients for humans. Therefore, in order to enhance the quality and production of milk in cattle, it is interesting to examine the underlying mechanisms. A number of new investigations and research have found that, circRNA; a specific class of non-coding RNAs, is linked with the development of mammary gland and lactation. In the present study, genome wide identification and expression of the circRNAs in mammary epithelial cells of two distinct cattle breeds viz Jersey and Kashmiri at peak lactation was conducted. We reported 1554 and 1286 circRNA in Jersey and Kashmiri cattle, respectively, with 21 circRNAs being differentially expressed in the two breeds. The developmental genes of the established differentially expressed circRNAs were found to be largely enriched in antioxidant activity, progesterone, estradiol, lipid, growth hormone, and drug response. Certain pathways like MAPK, IP3K and immune response pathways were found significantly enriched in KEGG analysis. These results add to our understanding of the controlling mechanisms connected with the lactation process, as well as the function of circRNAs in bovine milk synthesis. Additionally, the comparative analysis of differentially expressed circRNAs showed significant conservation across different species.

【 授权许可】

CC BY