LncRNA CCAT1 facilitates the proliferation, invasion and migration of human laryngeal squamous cell carcinoma cells via the miR-218-5p/BMI1

PeerJ

article

Jing Hong¹  Ali Hong²  Houshu Tu³  Zhichao Wan¹  Yuqiao Deng¹  Chengcheng Deng¹  Bo Tao¹  Yanjin Yu¹  Lanfei Zhou¹ 

[1] Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine;Jiangxi University of Traditional Chinese Medicine;Nanchang Angel Maternity Hospital

关键词: Laryngeal squamous cell carcinoma;    LncRNA CCAT1;    miR-218-5p;    BMI1;    Competing endogenous RNA;    Proliferation;    Migration;    Invasion;   

DOI  :  10.7717/peerj.12961

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Long non-coding RNAs (LncRNAs) are vital in the treatment of laryngeal squamous cell carcinoma (LSCC). This study estimated the mechanism of lncRNA CCAT1 (CCAT1) in LSCC cells. The expression of CCAT1 in the human laryngeal mucosal epithelial cells (HLCs) and LSCC cells (Hep-2 and TU177) was detected. CCK-8 and Transwell assays were used to evaluate the cell proliferative, migrative, and invasive abilities, respectively. The subcellular localization of CCAT1 was verified by RNA-FISH and cytoplasmic isolation assays. The targeted relationship among CCAT1, miR-218-5p, and BMI1 was verified by dual-luciferase assay. Expressions of miR-218-5p and BMI1 were detected by RT-qPCR. Our results depicted that CCAT1 was highly-expressed in Hep-2 and TU177 cells. Silencing CCAT1 inhibited the proliferation, migration, and invasion of Hep-2 and TU177 cells. Mechanically, CCAT1 regulated the BMI1 expression by competitively binding to miR-218-5p as a competing endogenous RNA (ceRNA), and thus facilitated the growth of Hep-2 and TU177 cells. Downregulation of miR-218-5p or upregulation of BMI1 inhibited the inhibitory effect of silencing CCAT1 on Hep-2 and TU177 cell proliferation, invasion, and migration. In conclusion, our study elicited that lncRNA CCAT1 facilitated the proliferation, migration, and invasion of Hep-2 and TU177 cells by sponging miR-218-5p and regulating the downstream BMI1.

【 授权许可】

CC BY