Whole-genome analysis of CGS , SAHH , SAMS gene families in five Rosaceae species and their expression analysis in Pyrus bretschneideri

PeerJ

article

Yang Zhang¹  Decong Li¹  Xiaofeng Feng¹  Xinya Wang¹  Mengna Wang¹  Wenlong Han¹  Muhammad Aamir Manzoor¹  Guohui Li²  Tianzhe Chen¹  Han Wang¹  Yongping Cai¹ 

[1] Anhui Agricultural University;West Anhui University

关键词: CGS;    SAHH;    SAMS;    Rosaceae;    Expression analysis;    Stone cell;   

DOI  :  10.7717/peerj.13086

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Cystathionine γ-synthase (CGS), S-adenosyl-L-homocysteine hydrolase (SAHH), and S-adenosy-L-methionine synthetase (SAMS) play an important role in the regulation of plant growth, development, and secondary metabolism. In this study, a total of 6 CGS, 6 SAHH, and 28 SAMS genes were identified from five Rosaceae species (Pyrus bretschneideri, Prunus persica, Prunus mume, Fragaria vesca, and Malus domestica). The evolutionary relationship and microsynteny analysis in five Rosaceae species revealed that duplicated regions were conserved between three gene families (CGS, SAHH, SAMS). Moreover, the chromosomal locations, gene structures, conserved motifs, cis-elements, physicochemical properties, and Ka/Ks analysis were performed by using numerous bioinformatics tools. The expression of different organs showed that the CGS, SAHH and SAMS genes of pear have relatively high expression patterns in flowers and stems, except for PbCGS1. RNA-seq and qRT-PCR combined analysis showed that PbSAMS1 may be involved in the regulation of pear stone cell development. In summary, this study provides the basic information of CGS, SAHH and SAMS genes in five Rosaceae species, further revealing the expression patterns in the pear fruit, which provides the theoretical basis for the regulation of pear stone cells.

【 授权许可】

CC BY