Comparative transcriptome analysis of cold-tolerant and -sensitive asparagus bean under chilling stress and recovery

PeerJ

article

Mingjun Miao¹  Huaqiang Tan³  Le Liang¹  Haitao Huang⁴  Wei Chang²  Jianwei Zhang¹  Ju Li²  Yi Tang¹  Zhi Li²  Yunsong Lai¹  Liang Yang²  Huanxiu Li¹ 

[1] College of Horticulture, Sichuan Agricultural University;Horticulture Research Institute, Sichuan Academy of Agricultural Sciences;Chengdu Academy of Agriculture and Forestry Sciences;Mianyang Academy of Agricultural Sciences

关键词: Asparagus bean;    Sesquipedialis;    RNA-seq;    Cold stress;    Recovery;    Transcriptome;   

DOI  :  10.7717/peerj.13167

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundLow temperature is a type of abiotic stress that threatens the growth and yield of asparagus bean. However, the key genes and regulatory pathways involved in low temperature response in this legume are still poorly understood. Methodology. The present study analyzed the transcriptome of seedlings from two asparagus bean cultivars—Dubai bean and Ningjiang 3—using Illumina RNA sequencing (RNA-seq). Correlations between samples were determined by calculating Pearson correlation coefficients (PCC) and principal component analysis (PCA). Differentially expressed genes (DEGs) between two samples were identified using the DESeq package. Transcription factors (TF) prediction, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs were also performed.Results0.88), decreasing to 0.79 and 0.81, respectively, at 12 and 24 h of recovery, consistent with the results of principal component analysis. The major transcription factor families identified from differentially expressed genes between the two cultivars included bHLH, NAC, C2H2, MYB, WRKY, and AP2/ERF. The representative GO enrichment terms were protein phosphorylation, photosynthesis, oxidation-reduction process, and cellular glucan metabolic process. Moreover, KEGG analysis of DEGs within each cultivar revealed 36 transcription factors enriched in Dubai bean and Ningjiang 3 seedlings under cold stress.ConclusionsThese results reveal new information that will improve our understanding of the molecular mechanisms underlying the cold stress response of asparagus bean and provide genetic resources for breeding cold-tolerant asparagus bean cultivars.

【 授权许可】

CC BY