Zonula occludens-1 expression is reduced in nasal epithelial cells of allergic rhinitis patients

PeerJ

article

Che Othman Siti Sarah¹  Siti Muhamad Nur Husna¹  Norasnieda Shukri²  Kah Keng Wong¹  Noor Suryani Mohd Ashari¹ 

[1] Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia;Department of Otorhinolaryngology, Head and Neck Surgery, School of Medical Sciences, Hospital Universiti Sains Malaysia, Universiti Sains Malaysia

关键词: Allergic rhinitis;    Zonula occludens;    Histone deacetylase;    House dust mites;    ZO-1;    ZO-2;    ZO-3;    HDAC1;    HDAC2;   

DOI  :  10.7717/peerj.13314

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Allergic rhinitis (AR) is a common allergic disease characterized by disruption of nasal epithelial barrier. In this study, we investigated the mRNA expression of zonula occludens-1 (ZO-1), ZO-2 and ZO-3 and histone deacetylase 1 (HDAC1) and HDAC2 in AR patients compared to healthy controls. RNA samples were extracted from nasal epithelial cells of house dust mites (HDMs)-sensitized AR patients and healthy controls (n = 28 in each group). The RNAs were reverse transcribed into cDNAs for measurement of ZO-1, ZO-2, ZO-3, HDAC1 and HDAC2 expression levels by quantitative PCR. The mRNA expression of ZO-1 was significantly decreased in AR patients compared to healthy controls (p = 0.010). No significant difference was observed in the expression levels of ZO-2, ZO-3, HDAC1 and HDAC2 in AR patients compared to healthy controls. We found significant associations of higher HDAC2 levels in AR patients with lower frequency of changing bedsheet (p = 0.043) and with AR patients sensitized to Dermatophagoides farinae (p = 0.041). Higher expression of ZO-2 was observed in AR patients who had pets (p = 0.007). In conclusion, our data indicated that ZO-1 expression was lower in AR patients contributing to decreased integrity of nasal epithelial barrier integrity, and HDAC2 may be involved in the pathogenesis of the disease.

【 授权许可】

CC BY