Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasis

PeerJ

article

Hua Wang¹  Chuan Jiang¹  Yakun Yang²  Jinghan Li¹  Yihan Wang³  Chaonan Wang¹  Yonggang Gao¹ 

[1] Hebei University of Chinese Medicine, Department of Preventive Medicine;Hebei University of Chinese Medicine, School of Pharmacy;Hebei University of Chinese Medicine, Collge of Basic Medicine;Hebei Key laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease

关键词: Resveratrol;    Iron overload;    Liver fibrosis;    Antioxidant;    Iron homeostasis;   

DOI  :  10.7717/peerj.13592

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

This study is intended to explore the protective effects of resveratrol (RES) on iron overload-induced liver fibrosis and its mechanism. Iron dextran (50 mg/kg) was injected intraperitoneally in all groups except the control group. Mice in the L-RES, M-RES and H-RES groups were gavaged with RES solution at 25, 50 mg/kg and 100 mg/kg, respectively, 4 h before injection of iron dextran every day; mice in the deferoxamine (DFO) group were injected with DFO intraperitoneally (100 mg/kg); mice in the control group received isovolumetric saline. After seven weeks of RES administration, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities and liver hydroxyproline (Hyp) levels were reduced; the malondialdehyde (MDA) activities decreased and the levels of superoxide dismutase (SOD) and glutathione (GSH) were raised. Hematoxylin and eosin (H&E), Prussian, and Masson staining indicated that RES treatment could improve cell damage and reduce hepatic iron deposition and collagen deposition in iron-overload mice. The expression of Bcl-2 was increased, the expression levels of Bax and caspase-3 were decreased under RES treatment. Moreover, RES reduced the expression of hepcidin, ferritin (Ft), divalent metal transporter-1 (DMT-1), transferrin receptor-2 (TFR-2), and raised the expression of ferroprotein-1 (FPN-1). In conclusion, RES could ameliorate iron overload-induced liver fibrosis, and the potential mechanisms may be related to antioxidant, anti-inflammatory, anti-apoptotic, and more importantly, regulation of iron homeostasis by reducing iron uptake and increasing iron export.

【 授权许可】

CC BY