Characterization of LC-MS based urine metabolomics in healthy children and adults

PeerJ

article

Xiaoyan Liu¹  Xiaoyi Tian²  Shi Qinghong⁴  Haidan Sun¹  Li Jing¹  Xiaoyue Tang¹  Zhengguang Guo¹  Ying Liu²  Yan Wang²  Jie Ma²  Ren Na²  Chengyan He⁴  Wenqi Song²  Wei Sun¹ 

[1] Proteomics Research Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College;Department of Clinical Laboratory, National Center for Children’s Health, Beijing Children’s Hospital, Capital Medical University;Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang University & Capital Medical University;China-Japan Union Hospital of Jilin University

关键词: Metabolomics;    Urine;    Children;    Adults;    Characterization;   

DOI  :  10.7717/peerj.13545

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Previous studies reported that sex and age could influence urine metabolomics, which should be considered in biomarker discovery. As a consequence, for the baseline of urine metabolomics characteristics, it becomes critical to avoid confounding effects in clinical cohort studies. In this study, we provided a comprehensive lifespan characterization of urine metabolomics in a cohort of 348 healthy children and 315 adults, aged 1 to 78 years, using liquid chromatography coupled with high resolution mass spectrometry. Our results suggest that sex-dependent urine metabolites are much greater in adults than in children. The pantothenate and CoA biosynthesis and alanine metabolism pathways were enriched in early life. Androgen and estrogen metabolism showed high activity during adolescence and youth stages. Pyrimidine metabolism was enriched in the geriatric stage. Based on the above analysis, metabolomic characteristics of each age stage were provided. This work could help us understand the baseline of urine metabolism characteristics and contribute to further studies of clinical disease biomarker discovery.

【 授权许可】

CC BY