期刊论文详细信息
PeerJ
Effects of commercial beverages on the neurobehavioral motility of Caenorhabditis elegans
article
Wenjing Zhang1  Nan Zhang1  Shan Zheng1  Wei Zhang1  Jingjing Liu1  Liwei He1  Anastasia Ngozi Ezemaduka2  Guojun Li1  Junyu Ning1  Bo Xian4  Shan Gao1 
[1] Beijing Center for Disease Prevention and Control, Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning;Key Laboratory of Wetland Ecology and Environment, Northeast institute of Geography and Agroecology, Chinese Academy of Sciences;School of Public Health, Capital Medical University;School of Medicine, University of Electronic Science and Technology of China
关键词: Beverage;    C. elegans;    Nematode;    Behavior;    Feeding;   
DOI  :  10.7717/peerj.13563
学科分类:社会科学、人文和艺术(综合)
来源: Inra
PDF
【 摘 要 】

To study the effects of different types of commercially available drinks/beverages on neurobehavior using the model organism C. elegans, and critically review their potential health hazards. Eighteen kinds of beverages from the supermarket were randomly selected and grouped into seven categories namely functional beverage, tea beverage, plant protein beverage, fruit juice beverage, dairy beverage, carbonated beverage and coffee beverage. The pH value, specific gravity and osmotic pressure were also examined. The L4 stage N2 worms were exposed to different concentration of tested beverages (0, 62.5, 125, 250 and 500 µL/mL) for 24 h to measure the survival rate and locomotory behavior such as head thrashing, body bending as well as pharyngeal pumping. All the 18 beverages tested did not induce any visible lethal effects in the nematodes. However, exposure to different types of tested beverages exhibited different effects on the behavioral ability of C. elegans: (1) sports functional beverage and herbal tea drink accelerated the head thrashing and body bending of nematodes when compared to the control group (P < 0.05). (2) The vibration frequency of the pharyngeal pump of nematodes was significantly accelerated after treated with three plant protein beverages (almond milk, coconut milk and milk tea) and dairy products A and B (P < 0.05), and decelerated after treatment with other tested beverages. (3) Carbonated beverage significantly inhibits the head thrashing, body bending and pharyngeal pumping vibration (P < 0.05). Our results indicate that 18 kinds of popular beverages in the market have different influence on the neurobehavior in C. elegans, which may be related to their different components or properties. Further research would be required to conduct a systematic analysis of the effect of beverages by appropriate kinds, taking into consideration other endpoints such as reproduction, lifespan and molecular stress response, etc., and to elucidate the mechanism for its potential health hazards.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO202307100003735ZK.pdf 3524KB PDF download
  文献评价指标  
  下载次数:12次 浏览次数:2次