Prevalence, type of infections and comparative analysis of detection techniques of intestinal parasites in the province of Belgarn, Saudi Arabia

PeerJ

article

Abdulrahman S. Alqarni¹  Majed H. Wakid²  Hattan S. Gattan² 

[1] Health Affairs, Sabt Al-Alaya General Hospital, Directorate of Health Affairs, Ministry of Health;Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University;Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University

关键词: Prevalence;    Intestinal parasites;    Protozoa;    Detection techniques;    Microscopy;    RDTs;    Real-time PCR;    Food handlers;    Belgarn;    Saudi Arabia;   

DOI  :  10.7717/peerj.13889

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe study was conducted to observe the prevalence and type of infection caused by intestinal parasites and to compare the techniques that are available for the detection.MethodA total of 112 stool samples were obtained from study participants, and the laboratory examinations were performed at Special Infectious Agents Unit, King Abdulaziz University, Jeddah. One hundred and twelve participants were included in this study.ResultThe color of positive specimens was mainly brown (86.4%). Stool consistency in infected cases was soft in (64%) samples. There was no statistically significant difference with the physical characteristics of the stool (P 0.05). In total, 59 of the 112 participants were infected with intestinal parasites, representing 52.7%. Different intestinal protozoa parasites were identified in which Blastocystis hominis (86.4%) was highest. None of the intestinal helminths were detected. Out of the 59 infected cases, single infections were found in (62.7%) samples. The intestinal protozoan parasites in single infections were B. hominis (78.4%), Giardia lamblia (8.1%), and (2.7%) from each Entamoeba histolytica, Cryptosporidium parvum, Entamoeba coli, Endolimax nana, and Chilomastix mesnili. Microscopy, RDTs, and real-time PCR were used for detection and identification of G. lamblia, E. histolytica, and C. parvum.ConclusionThe study reported a high rate of intestinal parasitic infections, mainly with B. hominis. There were no statistical differences in parasite detection between the three techniques in detection of the thirteen cases infected with the pathogenic protozoa.

【 授权许可】

CC BY