C1orf74 positively regulates the EGFR/AKT/mTORC1 signaling in lung adenocarcinoma cells

PeerJ

article

Jinyong Guo¹  Aili Li¹  Ruolin Guo¹  Qiufeng He¹  Youru Wu¹  Yi Gou¹  Junfei Jin²  Guojin Huang¹ 

[1] Laboratory of Respiratory Diseases, The Affiliated Hospital of Guilin Medical University;Molecular Medicine in Liver Injury and Repair, The Affiliated Hospital of Guilin Medical University;Guangxi Health Commission Key Laboratory of Basic Research in Sphingolipid Metabolism Related Diseases, The Affiliated Hospital of Guilin Medical University;China-USA Lipids in Health and Disease Research Center, Guilin Medical University

关键词: C1orf74;    Lung adenocarcinoma;    EGFR/AKT/mTORC1 signaling;    A549;    H1993;    HCC827;   

DOI  :  10.7717/peerj.13908

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Lung adenocarcinoma (LUAD) is a major type of lung cancer with poor prognosis and low 5-year survival rate, which urgently needs further investigation in order to elucidate its mechanisms completely and discover novel therapeutic targets. C1orf74 is a novel protein with unknown function either in normal cells or cancer cells. The aim of this study is to investigate the expression and function of C1orf74 in LUAD cells. Methods The expression of C1orf74 in LUAD was analyzed using the LUAD datasets from public databases. The prognostic value of C1orf74 in LUAD was analyzed using Kaplan-Meier Plotter. C1orf74 expression in LUAD cell line A549, H1993 and HCC827 was silenced using small interfering RNA, and then the effects of C1orf74 knockdown on proliferation, migration and invasion of LUAD cells were detected by colony formation assay and Transwell assay, the role of C1orf74 in EGFR/AKT/mTORC1 signaling pathway was examined by Western blot, and the function of C1orf74 in cell cycle was detected by flow cytometry. Results The results of LUAD clinical data showed that C1orf74 was upregulated in LUAD tissues, and its high expression was associated with poor prognosis. The results from cultured LUAD cells demonstrated that C1orf74 knockdown inhibited cell proliferation, migration and invasion, but induced cell cycle arrest and autophagy. Moreover, C1orf74 knockdown suppressed EGFR/AKT/mTORC1 signaling in LUAD cells. In conclusion, the present study revealed that C1orf74 is upregulated in LUAD tissues and plays an oncogenic role in LUAD, and that C1orf74 positively regulates cell proliferation and mobility through the EGFR/AKT/mTORC1 signaling pathway in LUAD cells.

【 授权许可】

CC BY