BMSC-derived exosomal miR-27a-3p and miR-196b-5p regulate bone remodeling in ovariectomized rats

PeerJ

article

Guohua Lai¹  Renli Zhao¹  Weida Zhuang¹  Zuoxu Hou¹  Zefeng Yang¹  Peipei He¹  Jiachang Wu¹  Hongxun Sang¹ 

[1] Department of Orthopedics, Shenzhen Hospital, Southern Medical University;The Third School of Clinical Medicine, Southern Medical University

关键词: Exosomes;    MicroRNAs;    Bone marrow mesenchymal stem cell;    Postmenopausal osteoporosis;    Osteogenic differentiation;    Osteoclast;    Next-generation sequencing;   

DOI  :  10.7717/peerj.13744

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundIn the bone marrow microenvironment of postmenopausal osteoporosis (PMOP), bone marrow mesenchymal stem cell (BMSC)-derived exosomal miRNAs play an important role in bone formation and bone resorption, although the pathogenesis has yet to be clarified.MethodsBMSC-derived exosomes from ovariectomized rats (OVX-Exo) and sham-operated rats (Sham-Exo) were co-cultured with bone marrow-derived macrophages to study their effects on osteoclast differentiation. Next-generation sequencing was utilized to identify the differentially expressed miRNAs (DE-miRNAs) between OVX-Exo and Sham-Exo, while target genes were analyzed using bioinformatics. The regulatory effects of miR-27a-3p and miR-196b-5p on osteogenic differentiation of BMSCs and osteoclast differentiation were verified by gain-of-function and loss-of-function analyses.ResultsOsteoclast differentiation was significantly enhanced in the OVX-Exo treatment group compared to the Sham-Exo group. Twenty DE-miRNAs were identified between OVX-Exo and Sham-Exo, among which miR-27a-3p and miR-196b-5p promoted the expressions of osteogenic differentiation markers in BMSCs. In contrast, knockdown of miR-27a-3p and miR-196b-5p increased the expressions of osteoclastic markers in osteoclast. These 20 DE-miRNAs were found to target 11435 mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these target genes were involved in several biological processes and osteoporosis-related signaling pathways.ConclusionBMSC-derived exosomal miR-27a-3p and miR-196b-5p may play a positive regulatory role in bone remodeling.

【 授权许可】

CC BY