Antimicrobial and cytotoxic effects of marine sponge extracts Agelas clathrodes , Desmapsamma anchorata and Verongula rigida from a Caribbean Island

PeerJ

article

Julie Piron¹  Stephane Betzi²  Jessica Pastour¹  Audrey Restouin²  Rémy Castellano²  Yves Collette²  Niklas Tysklind³  Juliette Smith-Ravin¹  Fabienne Priam¹ 

[1] Groupe de Recherche BIOSPHERES, Université des Antilles, Campus de Schoelcher;Centre de Recherche en Cancérologie de Marseille ,(CRCM) - Aix-Marseille Université, Inserm, CNRS, Institut Paoli Calmettes;INRAE - UMR 0745 ECOFOG, Campus Agronomique CEDEX;Association AREBio Immeuble Bellevue

关键词: Marine sponges;    Antimicrobial activity;    Cytotoxic activity;    Agelas clathrodes;    Desmapsamma anchorata;    Verongula rigida;    Natural products;    Martinique;    Tumoral cell lines;   

DOI  :  10.7717/peerj.13955

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Although marine sponges are known for their antimicrobial, antifungal and cytotoxic activity, very few studies have been carried out on endemic species of Martinique. Martinique is part of the Agoa Sanctuary, a marine protected area that includes the exclusive economic zones (EEZ) of the French Caribbean islands, making it an abundant source of marine species. To highlight the potential of this area for the discovery of marine biomolecules with antipathogenic and antitumor activities, we tested the aqueous and ethanolic extracts of sponge species Agelas clathrodes, Desmapsamma anchorata and Verongula rigida. Five bacterial strains: Bacillus cereus (CIP 78.3), Escherichia coli (CIP 54.127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125) were evaluated, as well as four tumor cell lines: breast cancer (MDA-MB231), glioblastoma (RES259) and leukemia (MOLM14 and HL-60). Antimicrobial activity was evaluated using the disc diffusion technique by determining the minimum inhibitory and minimum bactericidal concentrations. Tumor cytotoxic activity was determined in vitro by defining the minimum concentration of extracts that would inhibit cell growth. Ethanolic extracts of Agelas clathrodes were bactericidal for Staphylococcus aureus and Staphylococcus saprophyticus strains, as well as strongly cytotoxic (IC50 < 20 µg/mL) on all cancer cell lines. Verongula rigida also showed strong cytotoxic activity on cell lines but no antimicrobial activity. These results are innovative for this species on these bacterial lines, highlighting the potential of sponge extracts from this area as bioactive compounds sources.

【 授权许可】

CC BY