Irradiation with carbon ion beams affects soybean nutritional quality in early generations

PeerJ

article

Changkai Liu¹  Xue Wang¹  Yansheng Li¹  Heng Chen¹  Qiuying Zhang¹  Xiaobing Liu¹ 

[1]Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology

[2]University of Chinese Academy of Sciences

[3]Innovation Academy for Seed Design

关键词: Irradiation;    Soybean;    Protein;    Oil;    Isoflavones;    Carbohydrates;    Trace elements;   

DOI  :  10.7717/peerj.14080

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

As people’s demand for healthy diet increases, improving soybean seed nutritional quality is becoming as important as yield. Carbon ion beam radiation (CIBR) is an effective method to create soybean mutants, and thus breeding cultivars with better seed nutritional quality. In this study, the high-yield soybean line ‘Dongsheng 28’ was used, and three CIBR doses (100, 120, and 140 Gy) were used to explore the characteristics of quality separation and variation in the offspring of early mutant populations. Eleven quality traits, including protein, oil, sucrose, soluble sugar, iron (Fe), manganese (Mn), zinc (Zn), cupper (Cu), daidzin, glycitin, and genistin concentrations were analyzed in the M2 and M3 generations. The results revealed that the range of protein and oil concentration of all three CIBR doses changed by 38.5–42.9% and 18.8–23.8% in the M2 and M3 generations, respectively, while soluble sugar and sucrose concentrations changed by 48.1–123.4 and 22.7–74.7 mg/g, with significant effects by 140 Gy across the two generations. Therefore, around the optimum range, a higher CIBR dose is better for high protein, oil, and sugar varieties selection. In general, irradiation raised isoflavone concentrations, but 140 Gy had an inhibitory effect on isoflavone concentrations in the M3 generation. Although a variety could not be released in the M2 or M3 generation, the results of this study have important guiding significance for the targeted cultivation of specific nutritional quality materials. For instance, a lower irradiation dose is preferable when breeding targets are higher isoflavones and Mn concentrations. It is essential to increase the irradiation dose if the breeding targets contain high levels of protein, oil, sucrose, soluble sugars, Fe, Zn, and Cu.

【 授权许可】

CC BY