Virulence characteristics of Blumeria graminis f. sp. tritici and its genetic diversity by EST-SSR analyses

PeerJ

article

Yazhao Zhang¹  Xianxin Wu¹  Wanlin Wang¹  Yiwei Xu¹  Huiyan Sun¹  Yuanyin Cao¹  Tianya Li¹  Mansoor Karimi-Jashni² 

[1] College of Plant Protection, Shenyang Agricultural University;Department of Plant Pathology, Tarbiat Modares University

关键词: Blumeria graminis f. sp. tritici;    Wheat powdery mildew;    Resistance;    Pm genes;   

DOI  :  10.7717/peerj.14118

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (an obligate biotrophic pathogen) is a worldwide threat to wheat production that occurs over a wide geographic area in China. For monitoring genetic variation and virulence structure of Blumeria graminis f. sp. tritici in Liaoning, Heilongjiang, and Sichuan in 2015, 31 wheat lines with known Powdery mildew resistance genes and 2 EST-SSR markers were used to characterize the virulence and genetic diversity. Results indicated that 90% of all isolates were virulent on Pm3c, Pm3e, Pm3f, Pm4a, Pm5, Pm6 (Timgalen), Pm7, Pm16, Pm19, and Pm1 + 2 + 9 and 62.6% to 89.9% of isolates were virulent on Pm3a, Pm3b, Pm3d, Pm4b, Pm6 (Coker747), Pm8, Pm17, Pm20, Pm23, Pm30, Pm4 + 8, Pm5 + 6, Pm4b + mli, Pm2 + mld, Pm4 + 2X, Pm2 + 6. The Pm13 and PmXBD genes were effective against most collected isolates from Liaoning and Heilongjiang Provinces. Only Pm21 exhibited an immune infection response to all isolates. Furthermore, closely related isolates within each region were distinguished by cluster analyses using EST-SSR representing some gene exchanges and genetic relationships between the flora in Northeast China (Liaoning, Heilongjiang) and Sichuan. Only 45% of the isolates tested show a clear correlation between EST-SSR genetic polymorphisms and the frequency of virulence gene data. However, the EST-SSR polymorphism of isolated genes did not correspond to the virulence diversity of isolates in the single-gene lineage identification of hosts.

【 授权许可】

CC BY