Enhanced biomass and thermotolerance of Arabidopsis by SiERECTA isolated from Setaria italica L

PeerJ

article

Jiacheng Zheng¹  Xiaoyi Huang¹  Jieqin Li¹  Qingyuan He¹  Wan Zhao²  Chaowu Zeng³  Haizhou Chen⁴  Qiuwen Zhan¹  Zhaoshi Xu² 

[1] Anhui Science and Technology University, College of Agronomy;Chinese Academy of Agricultural Sciences ,(CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science;Xinjiang Academy of Agricultural Sciences, Institute of Crop Sciences;Anhui Youxin Agricultural Science and Technology Co. LTD

关键词: SiERECTA family;    Expression characteristics;    Thermotolerance;    Biomass;    Foxtail millet;   

DOI  :  10.7717/peerj.14452

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Foxtail millet is commonly used as a food and forage grass. ERECTA (ER) is a receptor-like kinase that can improve plant biomass and stress resistance. The sorghum SbER10_X1 gene was used as a probe to identify ER family genes on the Setaria italica genomes (SiERs), and determine the characteristics of the SiERs family. Herein, the structural features, expression patterns, and thermotolerance of SiERs function were identified by bioinformatics analysis, real-time PCR and transgenesis estimation. Results showed that SiERs had four members: two members were located on chromosome 1 with a total of six copies (SiER1_X1, SiER1_X2, SiER1_X3, SiER1_X4, SiER1_X5, and SiER1_X6), and two were on chromosome 4, namely, SiER4 (SiER4_X1 and SiER4_X2) and SiERL1. Among them, SiER1_X4 and SiER4_X1 were expressed highest in above-ground organs of foxtail millet, and actively responded to treatments with abscisic acid, brassinolide, gibberellin, and indole acetic acid. After overexpression of SiER1_X4 and SiER4_X1 in Arabidopsis, the plant height and biomass of the transgenic Arabidopsis significantly increased. Following high-temperature treatment, transgenic seedlings survived better compared to wild type. Transgenic lines showed higher SOD and POD activities, and expression level of AtHSF1 and AtBl1 genes significantly increased. These results indicated that SiER1_X4 and SiER4_X1 played important regulatory roles in plant growth and thermotolerance. The two genes provide potential targets for conventional breeding or biotechnological intervention to improve the biomass of forage grass and thermotolerance of field crops.

【 授权许可】

CC BY