The long-term dynamics of serum antibodies against SARS-CoV-2

PeerJ

article

Graziele Fonseca de Sousa¹  Thuany da Silva Nogueira¹  Lana Soares de Sales¹  Fernanda Ferreira Maissner¹  Odara Araújo de Oliveira¹  Hellade Lopes Rangel¹  Daniele das Graças dos Santos¹  Rodrigo Nunes-da-Fonseca¹  Jackson de Souza-Menezes¹  Jose Luciano Nepomuceno-Silva¹  Flávia Borges Mury¹  Raquel de Souza Gestinari¹  Amilcar Tanuri²  Orlando da Costa Ferreira Jr²  Cintia Monteiro-de-Barros¹ 

[1] Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro;Laboratório de Virologia Molecular, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro

关键词: S-UFRJ;    COVID-19 patients;    SARS-CoV-2-specific antibodies;    Spike glycoprotein;    Antibody production;    Serologic response;    Seroconversion;    Immune responses;    Age;    Gender;   

DOI  :  10.7717/peerj.14547

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Objective To analyze the long-term dynamics of antibodies against SARS-CoV-2 and understand the impact of age, gender, and viral load on patients’ immunological response. Methods Serum samples were obtained from 231 COVID-19 positive patients from Macaé, in Rio de Janeiro state, in Brazil, from June 2020 until January 2021. The production of IgA, IgM, IgG, and IgE against S glycoprotein was analyzed using the S-UFRJ assay, taking into account the age, gender, and viral load. Results Analysis of antibody production over 7 months revealed that IgA positivity gradually decreased after the first month. Additionally, the highest percentage of IgM positivity occurred in the first month (97% of patients), and declined after this period, while IgG positivity remained homogeneous for all 7 months. The same analysis for IgE revealed that almost all samples were negative. The comparison of antibody production between genders showed no significant difference. Regarding the age factor and antibody production, patients aged ≥60 years produced almost twice more IgA than younger ones (17–39 years old). Finally, a relationship between viral load and antibody production was observed only for older patients. Conclusions Our work provides an overview of long-term production of antibodies against SARS-CoV-2, suggesting prolonged production of IgA and IgM antibodies for 3 months and continued IgG production for over 7 months. In addition, it identified a correlation between viral load and IgM titers in the older group and, finally, different IgA production between the age groups.

【 授权许可】

CC BY