Co-circulation of all the four Dengue virus serotypes during 2018–2019: first report from Eastern Uttar Pradesh, India

PeerJ

article

Sthita Pragnya Behera¹  Pooja Bhardwaj¹  Hirawati Deval¹  Neha Srivastava¹  Rajeev Singh¹  Brij Ranjan Misra¹  Awdhesh Agrawal²  Asif Kavathekar¹  Rajni Kant¹ 

[1] ICMR-Regional Medical Research Centre;Division of Pathology, Gorakhnath Hospital

关键词: Gorakhpur;    Dengue;    Serotype;    E-UP;    Genotype;    Phylogeny;   

DOI  :  10.7717/peerj.14504

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Dengue fever is an endemic disease in India, transmitted by an infected mosquito bite. In India, the number of concurrent infections and the circulation of multiple dengue virus (DENV) serotypes has increased in recent decades. Molecular surveillance among the DENV serotype is important to keep track of the circulating serotypes, evolutionary changes, and key mutations that can alter the diagnostics. The current study included patients admitted for dengue in the Eastern Uttar Pradesh (E-UP) region during 2018–2019. The genetic characterization of the circulating DENV was accomplished through partial CprM (511 bp) gene amplification via reverse transcriptase polymerase chain reaction and sequencing. Phylogenetic analysis revealed the circulation of all four DENV1-4 serotypes. DENV-2 was the most abundant serotype (44%, 27/61), followed by DENV-3 (32%, 20/61). DENV-1 had a 16% (10/61) predominance, while DENV-4 (6%, 4/61) was found to be the least abundant serotype. DENV-2 genotypes were distributed among lineages I (7.4%), II (85%) and III (7.4%) of genotype IV, DENV-3 to lineage III of genotype III, DENV-1 to genotype III; DENV-2 to lineage B (75%) and C (25%) of genotype I. This primary report on the co-circulation of DENV1-4 serotypes from the E-UP region highlights the requirement of continuous molecular surveillance for monitoring circulating DENV serotypes.

【 授权许可】

CC BY