Influence of resin modified glass ionomer cement incorporating protein-repellent and antimicrobial agents on supragingival microbiome around brackets: an in-vivo split-mouth 3-month study

PeerJ

article

Yansong Ma¹  Chengjun Su¹  Hao Yang¹  Hockin H.K. Xu²  Yuxing Bai¹  Yan Xu¹  Xiaoxia Che¹  Ning Zhang¹ 

[1] Department of Orthodontics, School of Stomatology, Capital Medical University;Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine;Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine;Department of Advanced Oral Sciences and Therapeutics, School of Dentistry, University of Maryland

关键词: Orthodontic adhesive;    Oral microbiome;    Protein repellent;    Antimicrobial activity;    Enamel demineralization;   

DOI  :  10.7717/peerj.14820

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

ObjectiveTo explore the influence of resin modified glass ionomer cement (RMGIC) adhesives containing protein-repellent and quaternary ammonium salt agents on supragingival microbiome, enamel and gingival health around brackets.Materials and MethodsTen patients (21.4 ± 3.5 years) about to receive fixed orthodontics were enrolled in this study. Unilateral upper teeth bonded with RMGIC incorporating 2-Methacryloyloxyethyl phosphorylcholine (MPC) and Dimethylaminohexadecyl methacrylate (DMAHDM) were regarded as experimental group (RMD), while contralateral upper teeth bonded with RMGIC were control group (RMGIC), using a split-mouth design. Supragingival plaque was collected from both groups before treatment (T0), and at 1 month (T1) and 3 months (T2) of treatment. High-throughput sequencing was performed targeting v3–v4 of 16S rRNA gene. Streptococcus mutans and Fusobacterium nucleatum quantification was done by qPCR analysis. Bracket failures, enamel decalcification index (EDI), DIAGNODent scores (Dd), plaque index (PI) and gingival index (GI) were monitored at indicated time points.ResultsWithin 3 months, alpha and beta diversity of supragingival plaque had no difference between RMGIC and RMD groups. From T0 to T2, the relative abundance of Streptococcus depleted in RMD but remained steady in RMGIC group. Streptococcus, Prevotella, and Fusobacterium became depleted in RMD, Haemophilus and Capnocytophaga became depleted in RMGIC group but Prevotella enriched. Quantification of Fusbacterium nucleatum and Streptococcus mutans showed significant difference between RMGIC and RMD groups at T2. Teeth bonded with RMD had significant lower plaque index (PI) and DIAGNODent (Dd) score at T2, compared with teeth bonded with RMGIC (p < 0.05). No difference in bracket failure rate was examined between both groups (p 0.05).ConclusionBy incorporating MPC and DMAHDM into RMGIC, the material could affect the supragingival microbial composition, inhibit the progress of plaque accumulation as well as the key pathogens S. mutans and F. nucleatum in the early stage of orthodontic treatment.

【 授权许可】

CC BY