Effect of sulfur on pollen germination of Clemenules mandarin and Nova tangelo

PeerJ

article

Roberto Beltrán¹  Nuria Cebrián¹  Carlos Zornoza¹  Francisco García Breijo¹  José Reig Armiñana²  Alfonso Garmendia³  Hugo Merle¹ 

[1] Departamento de Ecosistemas Agroforestales, Universitat Politècnica de València;Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universidad de Valencia;Instituto Agroforestal Mediterráneo, Universitat Politècnica de València

关键词: Copper sulfate;    Ammonium sulfate;    Pollination;    Pollen tube;    Mandarin;    Inorganic sulfur;   

DOI  :  10.7717/peerj.14775

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

This study aims to elucidate whether sulfur can inhibit citrus pollination by affecting pollen grains. For this, four sulfur-based products (inorganic sulfur, water dispersible granular sulfur, ammonium sulfate, copper sulfate) were tested to evaluate their effect on pollen germination and pollen tube growth of two citrus varieties: Clemenules mandarin (Citrus clementina) and Nova tangelo (Citrus clementina x [Citrus paradisi x Citrus reticulata]). Pollen grains were extracted from the flowers of these two varieties and subsequently placed in Petri dishes with modified BK (boron and potassium) germination medium with six concentrations of the sulfur-based products (0.2, 2, 20, 200, 2,000, 20,000 mg l−1). All the dishes were incubated and the pollen germination rate was calculated. All the sulfur products showed progressive pollen germination inhibition with a rising sulfur concentration. CTC50 (50% cytotoxicity inhibition) was around 20 mg l−1, with significant differences among treatments. Total pollen germination inhibition took place at 20,000 mg l−1. These results demonstrate that sulfur application can affect citrus pollination.

【 授权许可】

CC BY