Transcriptome analysis of transcription factors and enzymes involved in monoterpenoid biosynthesis in different chemotypes of Mentha haplocalyx Briq

PeerJ

article

Xin An¹  Jingqiong Wan²  Hui Jiang²  Yangzhen Liao¹  Chang Liu³  Yuan Wei²  Chongwei Wen²  Zhen Ouyang¹ 

[1] School of Food and Biological Engineering, Jiangsu University;School of Pharmacy, Jiangsu University;Nanjing Institute for Comprehensive Utilization of Wild Plants

关键词: Mentha haplocalyx Briq;    Chemotype;    Transcriptome sequencing;    Monoterpenoid biosynthesis;    Transcription factor;    Regulation;   

DOI  :  10.7717/peerj.14914

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background The main active ingredients of Mentha haplocalyx Briq. essential oils are monoterpenes. According to the component of essential oils, M. haplocalyx can be divided into different chemotypes. Chemotype variation is widespread in Mentha plants but its formation mechanism is unclear. Methods We selected the stable chemotype l-menthol, pulegone, and carvone of M. haplocalyx for transcriptome sequencing. To further investigate the variation of chemotypes, we analyzed the correlation between differential transcription factors (TFs) and key enzymes. Results Fourteen unigenes related to monoterpenoid biosynthesis were identified, among which (+)-pulegone reductase (PR) and (−)-menthol dehydrogenase (MD) were significantly upregulated in l-menthol chemotype and (−)-limonene 6-hydroxylase was significantly upregulated in carvone chemotype. In addition, 2,599 TFs from 66 families were identified from transcriptome data and the differential TFs included 113 TFs from 34 families. The families of bHLH, bZIP, AP2/ERF, MYB, and WRKY were highly correlated with the key enzymes PR, MD, and (−)-limonene 3-hydroxylase (L3OH) in different M. haplocalyx chemotypes (r 0.85). The results indicate that these TFs regulate the variation of different chemotypes by regulating the expression patterns of PR, MD, and L3OH. The results of this study provide a basis for revealing the molecular mechanism of the formation of different chemotypes and offer strategies for effective breeding and metabolic engineering of different chemotypes in M. haplocalyx.

【 授权许可】

CC BY