Hydroxy proline and gamma-aminobutyric acid: markers of susceptibility to vine decline disease caused by the fungus Monosporascus cannonballus in melons ( Cucumis melo L.)

PeerJ

article

Sixto Alberto Marquez¹  Kevin Crosby¹  Bhimanagouda Patil¹  Carlos Avila³  Amir MH Ibrahim²  Herika Pessoa⁷  Jashbir Singh¹ 

[1] Department of Horticultural Sciences, Texas A&M University, College Station;Department of Horticultural Sciences, Texas A&M University, USDA National Center of Excellence for Melon at the Vegetable and Fruit Improvement Center, College Station;Department of Horticultural Sciences, Texas A&M University, Vegetable and Fruit Improvement Center, College Station;Department of Food Science and Technology, Texas A&M University, College Station;Department of Horticultural Sciences,Texas A&M Agrilife Research and Extension Center, Texas A&M University;Department of Soil and Crop Sciences, Texas A&M University, College Station;Agronomia, Universidad Federal de Viçosa-UFV

关键词: Vine Decline Disease;    Monosporascus cannonballus;    Hydroxy proline;    Gamma-aminobutyric acid;    Markers of susceptibility;    Melons;   

DOI  :  10.7717/peerj.14932

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundVine decline disease caused by the fungus Monosporascus cannonballus, is a threat to melon production (Cucumis melo L.) worldwide. Nonetheless, little is known about the metabolites produced during the host pathogen interaction. Thus, the objective of this study was to measure quantities of amino acids produced over time during such an interaction.MethodsTwo melon genotypes named TAM-Uvalde (susceptible) and USDA PI 124104 (resistant) were grown and inoculated with M. cannonballus. The metabolites previously stated were measured before inoculation (0 hours) and 24, 48 and 72 hours after inoculation, using high performance liquid chromatography analysis.ResultsThe production of some amino acids during the interaction of the resistant and susceptible melon genotypes with the fungus M. cannonballus was different regarding quantities over time. Interestingly, hydroxy proline was always up-regulated in higher quantities in response to pathogen infection in the genotype TAM-Uvalde. Also, the up-regulation in higher quantities of gamma-aminobutyric acid in the genotype TAM-Uvalde 48 and 72 hours after inoculation, suggests more penetration of the pathogen in its roots. Hence, taken together, hydroxy proline and gamma-aminobutyric acid levels could be used as markers of susceptibility to vine decline disease caused by M. cannonballus, which could be useful in developing resistant varieties.

【 授权许可】

CC BY