Biocontrol endophytes Bacillus subtilis R31 influence the quality, transcriptome and metabolome of sweet corn

PeerJ

article

Mingwei Shao¹  Yanhong Chen⁵  Qingyou Gong⁵  Shuang Miao¹  Chunji Li¹  Yunhao Sun¹  Di Qin¹  Xiaojian Guo¹  Xun Yan¹  Ping Cheng¹  Guohui Yu¹ 

[1] College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering;Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering;Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs;Guangdong University Key Laboratory for Sustainable Control of Fruit and Vegetable Diseases and Pests;Zhuhai Modern Agriculture Development Center

关键词: Biocontrol bacterium;    Bacillus subtilis R31;    Sweet corn;    Metabolomics;    Transcriptomics;   

DOI  :  10.7717/peerj.14967

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

During colonization of soil and plants, biocontrol bacteria can effectively regulate the physiological metabolism of plants and induce disease resistance. To illustrate the influence of Bacillus subtilis R31 on the quality, transcriptome and metabolome of sweet corn, field studies were conducted at a corn experimental base in Zhuhai City. The results show that, after application of B. subtilis R31, sweet corn was more fruitful, with a 18.3 cm ear length, 5.0 cm ear diameter, 0.4 bald head, 403.9 g fresh weight of single bud, 272.0 g net weight of single ear, and 16.5 kernels sweetness. Combined transcriptomic and metabolomic analyses indicate that differentially expressed genes related to plant-pathogen interactions, MAPK signaling pathway-plant, phenylpropanoid biosynthesis, and flavonoid biosynthesis were significantly enriched. Moreover, the 110 upregulated DAMs were mainly involved in the flavonoid biosynthesis and flavone and flavonol biosynthesis pathways. Our study provides a foundation for investigating the molecular mechanisms by which biocontrol bacteria enhance crop nutrition and taste through biological means or genetic engineering at the molecular level.

【 授权许可】

CC BY