Functional verification of the JmLFY gene associated with the flowering of Juglans mandshurica Maxim.

PeerJ

article

Jiayou Cai¹  Ruoxue Jia¹  Ying Jiang¹  Jingqi Fu¹  Tianyi Dong¹  Jifeng Deng¹  Lijie Zhang¹ 

[1] Shenyang Agricultural University;Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province

关键词: Juglans mandshurica Maxim.;    JmLFY gene;    Functional verification;   

DOI  :  10.7717/peerj.14938

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

In this study, a pBI121-JmLFY plant expression vector was constructed on the basis of obtaining the full-length sequence of the JmLFY gene from Juglans mandshurica, which was then used for genetic transformation via Agrobacterium inflorescence infection using wild-type Arabidopsis thaliana and lfy mutants as transgenic receptors. Seeds of positive A. thaliana plants with high expression of JmLFY were collected and sowed till the homozygous T3 regeneration plants were obtained. Then the expression of flowering-related genes (AtAP1, AtSOC1, AtFT and AtPI) in T3 generation plants were analyzed and the results showed that JmLFY gene overexpression promoted the expression of flowering-related genes and resulted in earlier flowering in A. thaliana. The A. thaliana plants of JmLFY-transformed and JmLFY-transformed lfy mutants appeared shorter leaves, longer fruit pods, and fewer cauline leaves than those of wild-type and the lfy mutants plants, respectively. In addition, some secondary branches in the transgenic plants converted into inflorescences, which indicated that the overexpression of JmLFY promoted the transition from vegetative growth to reproductive growth, and compensate the phenotypic defects of lfy mutant partially. The results provides a scientific reference for formulating reasonable genetic improvement strategies such as shortening childhood, improving yield and quality, and breeding desirable varieties, which have important guiding significance in production.

【 授权许可】

CC BY