Analysing transcriptomic signatures and identifying potential genes for the protective effect of inactivated COVID-19 vaccines

PeerJ

article

Hongquan Chen¹  Lu Zhang¹  Chen Xu¹  Xiaoyun Shen³  Jiazhou Lou¹  Shengjun Wu¹ 

[1] Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Zhejiang University;Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Zhejiang University;Key Laboratory of Endoscopic Technology Research, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Zhejiang University

关键词: SARS-CoV-2;    Inactivated vaccine;    RNA-seq;    Transcriptome profile;    Neutralization antibody;   

DOI  :  10.7717/peerj.15155

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Inactivated vaccines are one of the most effective strategies for controlling the coronavirus disease 2019 (COVID-19) pandemic. However, the response genes for the protective effect of inactivated vaccines are still unclear. Herein, we analysed the neutralization antibody responses elicited by vaccine serum and carried out transcriptome sequencing of RNAs isolated from the PBMCs of 29 medical staff receiving two doses of the CoronaVac vaccine. The results showed that SARS-CoV-2 neutralization antibody titers varied considerably among individuals, and revealed that many innate immune pathways were activated after vaccination. Furthermore, the blue module revealed that NRAS, YWHAB, SMARCA5, PPP1CC and CDC5L may be correlated with the protective effect of the inactivated vaccine. Additionally, MAPK1, CDC42, PPP2CA, EP300, YWHAZ and NRAS were demonstrated as the hub genes having a significant association with vaccines. These findings provide a basis for understanding the molecular mechanism of the host immune response induced by inactivated vaccines.

【 授权许可】

CC BY