An improved method for preparing stained ground teeth sections

PeerJ

article

Qizhong Qin¹  Yueheng Li²  Yujian Zhou¹ 

[1] Experimental Teaching and Management Center, Chongqing Medical University;Stomatological Hospital of Chongqing Medical University;College of Stomatology, Chongqing Medical University;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University

关键词: Ground sections of teeth;    Rosin;    Un-demineralization;   

DOI  :  10.7717/peerj.15240

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

ObjectiveIn oral histopathology teaching and research, there is a need for high-quality undemineralized tooth sections that are easy to handle, have controlled thickness, allow the observation of intact microstructures, and can be preserved for long periods of time.MethodsTeeth were collected under non-demineralizing conditions. Tooth sections (15–25 µm) were prepared using a diamond knife, then randomly divided into three groups: (1) stained with rosin, (2) stained with hematoxylin and eosin, or (3) not stained. The prepared tooth sections were evaluated by microscopy for clarity and microstructure visibility.ResultsThe use of a diamond knife in the sectioning and grinding process yielded high-quality ground sections of teeth. Rosin-stained ground sections allowed better identification of microstructures within the teeth, compared with unstained or hematoxylin and eosin-stained ground sections.ConclusionThe best results were obtained in the ground sections of teeth that were stained with rosin. Ground sections of teeth prepared using this staining method could be useful in oral histopathology teaching and research.

【 授权许可】

CC BY