Regenerative plantlets with improved agronomic characteristics caused by anther culture of tetraploid potato ( Solanum tuberosum L.)

PeerJ

article

Li Zhang¹  Feng-jie Nie¹  Lei Gong¹  Xiao-yan Gan¹  Guo-hui Zhang³  Xuan Liu¹  Wen-jing Yang¹  Lei Shi¹  Yu-chao Chen¹  Rui-xia Xie³  Zhi-qian Guo³  Yuxia Song¹ 

[1] Research Center of Agricultural Biotechnology, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan;College of Agriculture, Gansu Agricultural University;Guyuan Institute of Agricultural Sciences, Ningxia Academy of Agricultural and Forestry Sciences

关键词: Potato (Solanum tuberosum L.);    Anther culture;    Callus;    Regenerative;    Ploidy;    Morphological identification;   

DOI  :  10.7717/peerj.14984

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

ObjectiveAs the primary means of plant-induced haploid, anther culture is of great significance in quickly obtaining pure lines and significantly shortening the potato breeding cycle. Nevertheless, the methods of anther culture of tetraploid potato were still not well established.MethodsIn this study, 16 potato cultivars (lines) were used for anther culture in vitro. The corresponding relation between the different development stages of microspores and the external morphology of buds was investigated. A highly-efficient anther culture system of tetraploid potatoes was established.ResultsIt was shown in the results that the combined use of 0.5 mg/L 1-Naphthylacetic acid (NAA), 1.0 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D), and 1.0 mg/L Kinetin (KT) was the ideal choice of hormone pairing for anther callus. Ten of the 16 potato cultivars examined could be induced callus with their respective anthers, and the induction rate ranged from 4.44% to 22.67% using this hormone combination. According to the outcome from the orthogonal design experiments of four kinds of appendages, we found that the medium with sucrose (40 g/L), AgNO3 (30 mg/L), activated carbon (3 g/L), potato extract (200 g/L) had a promotive induction effect on the anther callus. In contrast, adding 1 mg/L Zeatin (ZT) effectively facilitated callus differentiation.ConclusionFinally, 201 anther culture plantlets were differentiated from 10 potato cultivars. Among these, Qingshu 168 and Ningshu 15 had higher efficiency than anther culture. After identification by flow cytometry and fluorescence in situ hybridization, 10 haploid plantlets (5%), 177 tetraploids (88%), and 14 octoploids (7%) were obtained. Some premium anther-cultured plantlets were further selected by morphological and agronomic comparison. Our findings provide important guidance for potato ploidy breeding.

【 授权许可】

CC BY