TMEM200A is a potential prognostic biomarker and correlated with immune infiltrates in gastric cancer

PeerJ

article

Fujin Fang¹  Tiantian Zhang³  Huan Lei¹  Xiaobing Shen¹ 

[1] Key Laboratory of Environmental Medical Engineering and Education Ministry, Southeast University;Department of Preventive Medicine, Southeast University;Department of Clinical Laboratory, The Third People’s Hospital of Bengbu

关键词: Gastric cancer;    TMEM200A;    TCGA;    Prognosis;    Immune infiltrates;   

DOI  :  10.7717/peerj.15613

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Gastric cancer (GC) is one of the most common malignant tumors in the digestive system. Several transmembrane (TMEM) proteins are defined as tumor suppressors or oncogenes. However, the role and underlying mechanism of TMEM200A in GC remain unclear. Methods We analyzed the expression of TMEM200A in GC. Furthermore, the influence of TMEM200A on survival of GC patients was evaluated. The correlations between the clinical information and TMEM200A expression were analyzed using chi-square test and logistic regression. Relevant prognostic factors were identified performing univariate and multivariate analysis. Gene set enrichment analysis (GSEA) was performed based on the TCGA dataset. Finally, we explore the relationship between TMEM200A expression and cancer immune infiltrates using CIBERSORT. Results TMEM200A was up-regulated in GC tissues than that in adjacent non-tumor tissues based on TCGA database. Meta-analysis and RT-qPCR validated the difference in TMEM200A expression. Kaplan-Meier curves suggested the increased TMEM200A had a poor prognosis in GC patients. The chi-square test and logistic regression analyses showed that the TMEM200A expression correlates significantly with T stage. Multivariate analysis showed that TMEM200A expression might be an important independent predictor of poor overall survival in GC patients. GSEA identified five immune-related signaling pathways and five tumor-related signaling pathways significantly enriched in the high TMEM200A expression phenotype pathway. Finally, we found CD8+ T cells is apparently decreased in high TMEM200A expression group. Conversely, eosinophils is increased in high expression group compared with low expression group. Conclusion TMEM200A is a potential prognostic biomarker and correlated with immune infiltrates in GC.

【 授权许可】

CC BY