Immunostimulation of Parasteatoda tepidariorum (Araneae: Theridiidae) in juvenile and adult stages. Immunity reactions to injury with foreign body and Bacillus subtilis infection

PeerJ

article

Agnieszka Ewa Czerwonka¹  Marta Katarzyna Sawadro¹  Jolanta Brożek¹  Agnieszka Izabela Babczyńska¹ 

[1] University of Silesia

关键词: Immunocompetence;    Invertebrate immunity;    Immunostimulants;    Bacterial infection;    Injury;    Spider;    Parasteatoda tepidariorum;    Immunostimulation;   

DOI  :  10.7717/peerj.15337

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

To assess the immune potential of spiders, in the present study juvenile and adult females of Parasteatoda tepidariorum were exposed to Bacillus subtilis infection, injury by a nylon monofilament and a combination of both. The expression level of selected immune-related genes: defensin 1 (PtDEF1), lysozyme 1 (PtLYS1), lysozyme C (PtLYSC), lysozyme M1 (PtLYSM1), autophagy-related protein 101 (PtATG101), dynamin (PtDYN) and heat shock proteins (HSP70) (PtHSPB, PtHSPB2A, PtHSPB2B), production of lysozyme and HSP70 proteins, and hemocytes viability were measured. The obtained results indicated expression of the lysozyme, autophagy-related protein and HSP70 genes in both ontogenetic stages of P. tepidariorum. It has been also shown that the simultaneous action of mechanical and biological factors causes higher level of lysozyme and HSP70, cell apoptosis intensity and lower level of hemocytes viability than in the case of exposure to a single immunostimulant. Moreover, mature females showed stronger early immune responses compared to juveniles.

【 授权许可】

CC BY