Protective effect of fustin against adjuvant-induced arthritis through the restoration of proinflammatory response and oxidative stress

PeerJ

article

Sultan Alshehri¹  Shareefa A. AlGhamdi²  Amira M. Alghamdi²  Syed Sarim Imam¹  Wael A. Mahdi¹  Mohammad A. Almaniea¹  Baraa Mohammed Hajjar¹  Fahad A. Al-Abbasi²  Nadeem Sayyed⁴  Imran Kazmi² 

[1] Department of Pharmaceutics, College of Pharmacy, King Saud University;Department of Biochemistry, Faculty of Sciences, King Abdulaziz University;Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University;Glocal School of Pharmacy, Glocal University

关键词: Fustin;    Arthritis;    Freund’s adjuvant;    Inflammatory cytokines;   

DOI  :  10.7717/peerj.15532

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Rheumatoid arthritis causes irreparable damage to joints. The present research sought to check fustin’s anti-arthritic efficacy against the complete Freund’s adjuvant-induced arthritis paradigm in animals by altering the inflammatory response. In the rats, complete Freund’s adjuvant was used to trigger arthritis and they received fustin at 50 and 100 mg/kg for 21 days. At regular intervals, the hind paw volume and arthritic score were assessed. After the trial period, hematological, antioxidant, pro-inflammatory cytokines, and other biochemical parameters were estimated. Fustin-treated rats showed the down-regulation of hind paw volume, arthritic score, and altered hematological parameters (TLC, DLC (neutrophil, lymphocyte, monocyte, eosinophil, basophil)). Furthermore, fustin significantly mitigates proinflammatory cytokine (reduced interleukin, tumor necrosis factor-a (TNF-α), IL-6, IL-1β), oxidative stress (attenuated malondialdehyde (MDA), catalase (CAT), glutathione (GSH), superoxide dismutase (SOD)), attenuated production of prostaglandin E2 and myeloperoxidase (MPO) and improved nuclear factor erythroid 2-related factor (Nrf2) action. Fustin led to the benefit in arthritis-prone animals elicited by complete Freund’s adjuvant via pro-inflammatory cytokine.

【 授权许可】

CC BY