Biological properties of mucus from land snails ( Lissachatina fulica ) and freshwater snails ( Pomacea canaliculata ) and histochemical study of mucous cells in their foot

PeerJ

article

Phornphan Phrompanya¹  Narinnida Suriyaruean¹  Nattawadee Nantarat¹  Supap Saenphet¹  Yingmanee Tragoolpua¹  Kanokporn Saenphet¹ 

[1] Department of Biology, Faculty of Science, Chiang Mai University;Ph.D.’s Degree Program in Biology ,(International Program), Faculty of Science, Chiang Mai University

关键词: Antioxidant;    Anti-inflammation;    Snail mucus;    Histochemical;    Mucous cell;    Lissachatina fulica;    Pomacea canaliculata;   

DOI  :  10.7717/peerj.15827

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundMucus derived from many land snails has been extensively utilised in medicine and cosmetics, but some biological activities of the mucus need to be well documented. Nevertheless, most mucus is obtained from land snails, while mucus from freshwater snails has yet to be attended.MethodsThis study aims to determine and compare mucus’s antioxidant and anti-inflammatory activities from the land snail Lissachatina fulica and the freshwater snail Pomacea canaliculata. ABTS, DPPH, reducing power and total antioxidant activity assays were used to evaluate the antioxidant capacity. Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 cells was performed to determine the anti-inflammatory activity. Additionally, the histochemical analysis of mucous cells in each snail foot was conducted to compare the distribution of mucous cells and types of mucins using periodic acid-Schiff and Alcian blue staining.ResultsMucus from L. fulica and P. canaliculata exhibited antioxidant and anti-inflammatory activities in different parameters. L. fulica mucus has higher total antioxidant (44.71 ± 2.11 mg AAE/g) and nitric oxide inhibitory activities (IC50 = 9.67 ± 0.31 µg/ml), whereas P. canaliculata mucus has better-reducing power activity (43.63 ± 2.47 mg AAE/g) and protein denaturation inhibition (IC50 = 0.60 ± 0.03 mg/ml). Histochemically, both species’ dorsal and ventral foot regions contained neutral and acid mucins in different quantities. In the dorsal region, the neutral mucins level in L. fulica (16.64 ± 3.46%) was significantly higher than that in P. canaliculata (11.19 ± 1.50%), while the acid mucins level showed no significant difference between species. Levels of both mucins in the ventral foot region of L. fulica (15.08 ± 3.97% and 10.76 ± 3.00%, respectively) were significantly higher than those of P. canaliculata (2.25 ± 0.48% and 2.71 ± 0.56%, respectively). This study revealed scientific evidence of the biological capacity of mucus from L. fulica and P. canaliculata as well as provided helpful information on the region of the foot which produces effective mucus.

【 授权许可】

CC BY