N-glycans Profiling in Pilocarpine Induced Status Epilepticus in Immature Rats

European Pharmaceutical Journal

article

S. Kapoor¹  M. Nemcovic³  J. Folbergrova²  D. Kala²  J. Svoboda²  J. Otahal²  Z. Brnoliakova¹ 

[1]Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences

[2]Institute of Physiology, Czech Academy of Sciences

[3]Institute of Chemistry, Slovak Academy of Sciences

关键词: N-glycans;    MALDI-TOF/MS;    Status epilepticus;    Sialic acid;    New-born rats;   

DOI  :  10.2478/afpuc-2022-0011

学科分类：社会科学、人文和艺术（综合）

来源: Walter de Gruyter GmbH

【 摘 要 】

Status epilepticus (SE) is a common neurological emergency in children and a well-known epileptogenic insult. Neonates are extremely susceptible to seizures in the neonatal period due to the higher vulnerability. Neonatal SE is associated with significant mortality and morbidity. There is an evident need for attention on neonatal SE in research due to the incredibly limited diagnostic and treatment options in current neonatology, and its serious long-term consequences. The aim of the present study was to characterize the glycoprofiles in the pilocarpine-induced SE model in immature rats to assess the overall N-glycans composition. To induce lithium-pilocarpine (Li-Pilo) SE male Wistar rat pups were pretreated with lithium chloride (127 mg/kg, n=11) on the 11th postnatal day. After 24 hours, the lithium pre-treated pups were administered either with pilocarpine intraperitoneally (i.p.) (35 kg/g, n=6) or saline (n=5) in the control group (Control). On the 19th postnatal day, serum was collected and the analytical procedures were done by mass spectrometry (MS) analytics on matrix-assisted laser desorption/ionization in combination with a time-of-flight detector (MALDI-TOF/MS). Analyzed data were processed by FlexAnalysis (Bruker Daltonics) and GlycoWorkbench software. There were 21 N-glycans that were identified, appointed, and sorted with special emphasis on their structure.

【 授权许可】

CC BY-NC-ND