Relaxant effect of bioactive component compatibility of San-ao decoction on In vitro guinea pig airway smooth muscle: A dose-response relationship study

World Journal of Traditional Chinese Medicine

article

Wen-Jie Song¹  Yan-Ling Fu²  Sheng-Lou Ni³  Jia-Jia Fan²  Qian Du⁴  Hao Zheng¹ 

[1] Chinese Medical College, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine;Chinese Medical College, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine;Periodicals Publishing Center, Beijing University of Chinese Medicine;Wenzhou Traditional Chinese Medicine Hospital

关键词: Airway smooth muscle;    bioactive component compatibility;    dose–response manner;    relaxant effect;    San-ao decoction;   

DOI  :  10.4103/wjtcm.wjtcm_64_21

学科分类：内科医学

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Background: Component compatibility is important to the modernization of traditional Chinese medicine. Studies have shown that San-ao decoction (SAD) can treat respiratory diseases by relaxing airway smooth muscle (ASM) and reducing airway hyper-responsiveness. However, whether its bioactive components and compatibility also present with similar relaxant effects remains unknown. This study aims to explore the potential relaxant property, dose-response relationship, and underlying mechanisms of the bioactive component compatibility in SAD. Methods: Network pharmacology was primarily used to identify the bioactive components of SAD and uncover its underlying mechanisms. ASM tension force measuring technique was utilized to verify the relaxant and dose-response effects on in vitro guinea pig ASM. Results: We postulated pseudoephedrine hydrochloride (PH), amygdalin (AM), and diammonium glycyrrhizate (DG) to be the bioactive components of SAD, which could effectively relax ASM in a dose-dependent manner on both acetylcholine-induced and spontaneous contraction. Both PH and AM could lead to DG dose–response curve shift. The regression equation of these three bioactive components was Y = −2.048 × X 1 + 0.411 × X 2 + 14.052 × X 3 (X 1 , X 2 , X 3 representing PH, AM, and DG, respectively). The underlying mechanisms of these components might be associated with the regulation of smooth muscle contraction. Conclusions: PH, AM, and DG are the bioactive components of SAD, which can relax ASM in a dose–response manner and exert a synergistic effect. Clinically, compatibility of these three bioactive components may serve as a new complementary and alternative treatment for respiratory diseases.

【 授权许可】

CC BY-NC-SA