Ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry based bile and urine metabonomics study on the ameliorative effects of Curcuma wenyujin rhizoma on acute blood stasis in rats

World Journal of Traditional Chinese Medicine

article

Min Hao¹  Meng-Ting Zhao²  Huang-Jin Tong³  De Ji⁴  Lin Li⁴  Lian-Lin Su⁴  Wei Gu⁴  Chun-Qin Mao⁴  Tu-Lin Lu⁴ 

[1] Chinese Medicine Processing Engineering Center, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University;Chinese Medicine Processing Laboratory, School of Pharmacy, Guizhou University of Traditional Chinese Medicine;Department of Pharmacy, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine;Chinese Medicine Processing Laboratory, College of Pharmacy, Nanjing University of Chinese Medicine

关键词: Bile;    Curcuma wenyujin rhizome;    metabolomics;    ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry;    urine;   

DOI  :  10.4103/wjtcm.wjtcm_55_21

学科分类：内科医学

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Background: Curcuma wenyujin rhizome (CWR) is a commonly used Chinese herbal medicine for treating blood stasis in China for 1000 of years. However, the underlying mechanism of CWR remains unclear. Aims and Objectives: The purpose of this study is to clarify the bioactive mechanism of CWR in treating blood stasis. Materials and Methods: In this study, pharmacological indexes, including hemorheology and four blood coagulation indexes were tested. Bile and urine metabolomics were engaged by UPLC-Q/TOF-MS. Multivariate statistical analysis were used to screen out differential endogenous metabolites. Results: The results indicated that CWR significantly ameliorated the hemorheology and coagulation functions of acute blood stasis (ABS) model rats. Moreover, 27 endogenous metabolites between the CWR group and the ABS group were screened, and the levels were all improved to certain degrees by CWR preadministration. Metabonomics results indicated that ABS was mainly related to linoleic acid metabolism, arachidonic acid metabolism, glycerophospholipid metabolism, sphingolipid metabolism, pentose and glucuronate intercereasonversions, steroid hormone biosynthesis, and primary bile acid biosynthesis. Conclusion: In a word, the metabolomics method is consistent with the holistic view of traditional Chinese medicine (TCM) that can be a powerful means to illustrate the biological activity mechanism of CWR in treating blood stasis and to offer research demonstration for further study on the effector mechanism of TCM.

【 授权许可】

CC BY-NC-SA