Semen collection methods and cooling rates affect post-thaw sperm motility and kinematic parameters of Saanen goat

Asian Pacific Journal of Reproduction

article

Lukusa, Kambulu¹  Kabuba, John² 

[1] Department of Animal & Wildlife Sciences, University of Pretoria, Private Bag X20;Department of Chemical Engineering, Vaal University of Technology, Private Bag X021

关键词: Artificial vagina;    Electro-ejaculator;    Sperm characteristics;    Cryopreservation;   

DOI  :  10.4103/2305-0500.294666

学科分类：兽医学

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Objective:  To evaluate the effects of semen collection methods and different cooling rates on cooled and frozen-thawed Saanen goat semen. Methods:  Twenty bucks were divided into two groups of 10 animals per group based on semen collection methods: artificial vagina and electro-ejaculator. Samples from each collection method were pooled and diluted with Tris-based extender. The pooled semen was divided into two cooling methods: slow cooling and fast cooling. Sperm motility and velocity, sperm plasma membrane integrity, sperm viability, mitochondrial membrane potential and DNA integrity were evaluated before dilution, immediately after cooling at 4 °C and at 24 h after freezing by using computer assisted sperm analyser method and epifluorescence microscope. Results:  Mean values for total motility, rapid-speed and progressive motile spermatozoa were significantly higher in semen collected with artificial vagina than electro-ejaculator (P<0.001). Slow cooling resulted in higher percentages of total motile, rapid-speed and progressive motile spermatozoa as compared to fast cooling (P<0.05). The post-thaw percentage of rapid-speed spermatozoa collected by artificial vagina in slow cooled sperm was significantly higher as compared to electro-ejaculator method (P<0.05). The mean values of curvilinear velocity and straight-line velocity were significantly higher in slow cooled semen obtained by artificial vagina (P<0.001). Similarly, the combination of artificial vagina and slow cooling resulted in significantly higher post-thaw percentages of straight-line velocity, average-path velocity, sperm plasma membrane integrity, viability and acrosome integrity (P<0.01). No significant differences were observed in slow cooling between artificial vagina and electro-ejaculator in terms of post-thaw sperm mitochondrial membrane potential and DNA integrity (P0.05). Conclusions:  The use of artificial vagina and slow cooling rate in cryopreservation protocol improves the cooled and post-thaw sperm quality of Saanen buck semen.

【 授权许可】

CC BY-NC-SA