Influence of N -acetylcysteine on pituitary-gonadal axis hormones and protamine expression level in streptozotocin-induced diabetic male rats

Asian Pacific Journal of Reproduction

article

Hassanzadeh Moghadam, Maryam¹  Shariati, Mehrdad³  Naeimi, Sirous⁴  Edalatmanesh, Mohammad Amin¹ 

[1] Department of Biology, Fars Science and Research Branch, Islamic Azad University;Department of Biology, Shiraz Branch, Islamic Azad University;Department of Biology, Kazerun Branch, Islamic Azad University;Department of Genetics, Kazerun Branch, Islamic Azad University

关键词: N-acetylcysteine;    Diabetes;    Testosterone;    Protamine;    Rat;   

DOI  :  10.4103/2305-0500.281258

学科分类：兽医学

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Objective:  To study the influence of N-acetylcysteine on the pituitary-gonadal axis hormones and protamine expression level in streptozotocin-induced diabetic male rats. Methods:  Forty-two adult male Wistar rats were divided into 6 groups, with 7 rats in each group. The control group left untreated; the streptozotocin group only received 50 mg/kg body weight streptozotocin intraperitoneally for 5 days to induce diabetes; the N-acetylcysteine group only received 200 mg/kg body weight N-acetylcysteine intraperitoneally, and the streptozotocin+N- acetylcysteine groups 1, 2 and 3 received 50 mg/kg streptozotocin intraperitoneally for 5 days to induce diabetes and then received 100, 200 and 400 mg/kg body weight doses of N-acetylcysteine intraperitoneally for 28 days, respectively. Enzyme-linked immunosorbent assay was used to measure the serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone, and real-time PCR was applied for measuring protamine expression level. Results:  Compared to the control and N-acetylcysteine groups, a significant decrease in the body weight, testicular weight and levels of testosterone and protamine expression was observed in the streptozotocin group and the streptozotocin+N-acetylcysteine groups 1 and 2. On the contrary, the levels of LH and FSH increased significantly. In the streptozotocin+N-acetylcysteine group 3, the body weight, testicular weight and expression level of protamine were significantly higher than those of the streptozotocin group. In the streptozotocin+N-acetylcysteine groups, testosterone and LH levels were significantly higher than and lower than the streptozotocin group, respectively. In the streptozotocin+N- acetylcysteine groups 2 and 3, the level of FSH was significantly lower than that of the streptozotocin group and streptozotocin+N- acetylcysteine group 1. Furthermore, a significant increase in the expression level of protamine was observed in the streptozotocin+N- acetylcysteine groups 2 and 3 when compared to the streptozotocin group and streptozotocin+N-acetylcysteine group 1. Conclusions:  N-acetylcysteine in an optimal dose of 400 mg/kg body weight has a protective influence on the pituitary-gonadal axis hormones and also on the expression level of protamine in diabetic male rats.

【 授权许可】

CC BY-NC-SA