Effect of short-term gavage of ethanolic extract of cogon grass ( Imperata cylindrica L) root on the ovarian activity and estrus behavior of female mice

Asian Pacific Journal of Reproduction

article

Widyastuti, Rini¹  Boediono, Arief³  Syamsunarno, Mas Rizky Anggun Adipurna²  Ghozali, Mohammad⁴  Ritonga, Mulyanusa Amarullah⁵  Lubis, Alkaustariyah⁶  Robianto, Sondi⁶  Sudiman, Jaqueline⁷ 

[1] Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran;Central Laboratory, Universitas Padjadjaran;Laboratory of Embryology, Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University;Department of Biomedical Science Faculty of Medicine, Universitas Padjadjaran;Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Padjadjaran, Hasan Sadikin Hospital;Study Program of Undergraduate Medicine, Faculty of Medicine, Universitas Padjadjaran;Department of Obstetrics and Gynaecology, Faculty of Medicine, Udayana University

关键词: Imperata cylindrica L;    Estrus cycle;    Ovary;    Endometrium;    Reproductive hormone;   

DOI  :  10.4103/2305-0500.281076

学科分类：兽医学

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Objective:  To assess the effect of short-term gavage of ethanolic extract of Imperata cylindrica L root on the ovarian activity and estrus behavior of female mice. Methods:  Eighteen virgin female ddY mice, 8 to 10 weeks of age, weighing 22–25 g with regular estrus cycle, were divided into three groups. Group 1 received 0.5% carboxymethylcellulose, whereas Groups 2 and 3 received the ethanolic extract of Imperata cylindrica L root at 90 and 115 mg/kg body weight (b.w.) per day by gavage for 20 days, respectively. All of the groups were checked before 9 a.m. daily for vaginal cytology to determine the estrus phase. On day 21, the mice were sacrificed to collect serum samples to quantify the concentrations of reproductive hormones using enzyme-linked immunosorbent assay and to determine changes in the reproductive organs based on their reproductive organ weight, histomorphology, and histomorphometry of ovarium and uterus. Results:  The reproductive organ weight in the treatment groups was similar compared with that in the control group. The 90 mg/kg b.w. treatment group showed an increase in corpus luteum number when compared with the control group, with few degenerated follicles and diminished oocytes. Moreover, the 115 mg/kg b.w. treatment group showed fewer primordial and primary follicles and an increase in corpus luteum number and a prolonged diestrus phase compared to the control and 90 mg/kg b.w. treatment groups. The histomorphology examination of the uterus showed that the thickness of myometrium and epithelium in the treated animals was similar to the control group. In addition, there was a significant decrease in follicle-stimulating hormone level in the 115 mg/kg b.w. treatment group (P<0.05). Conclusions:  Short-term gavage of ethanolic extract of Imperata cylindrica L root reduces the follicle-stimulating hormone serum level and folliculogenesis.

【 授权许可】

CC BY-NC-SA