Pertanika Journal of Tropical Agricultural Science | |
Screening of West Nile Virus, Herpesvirus, and Parvovirus in Rattus spp. in Klang Valley, Malaysia | |
article | |
Jafar Ali Natasha1  Abd Rahaman Yasmin1  Abd Manan Siti-Maisarah3  Zakaria Nur-Anis3  Murugasu Tharshaini1  Siti Suri Arshad3  Wan Noor Ayuni1  Mohammed Nma Mohammed1  Saulol Hamid Nur-Fazila3  | |
[1] Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine, Universiti Putra Malaysia;Laboratory of Vaccines and Immunotherapeutic, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaFaculty of Agricultural Product Innovation and Technology, Srinakharinwirot University;Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia | |
关键词: Herpesvirus; Klang Valley; parvovirus; Rattus spp.; virus; West Nile virus; | |
DOI : 10.47836/pjtas.45.4.15 | |
学科分类:农业科学(综合) | |
来源: Universiti Putra Malaysia, Universiti Pertanian Malaysia Press | |
【 摘 要 】
Free-roaming and scavenging lifestyles of Rattus spp. In densely populated urban areas expose them to multiple pathogens that facilitate the transmission of infection to the human population more rapidly, raising public health concerns. There is limited information on the status of rat susceptibility to virus infection, particularly West Nile virus (WNV), herpesvirus, and parvovirus, to prepare for emerging zoonosis. A total of 23 (n = 23) blood samples collected from Rattus spp. In the wet market areas of Klang Valley, Malaysia, were subjected to molecular assay using a one-step reverse transcription-polymerase chain reaction (RT-PCR) to detect the highly conserved region of the WNV capsid and pre-membrane protein via nested polymerase chain reaction (PCR) assay targeting highly conserved amino acid motifs within the herpesviral DNA-directed DNA polymerase gene (DPOL) and polymerase chain reaction (PCR) assay targeting the parvovirus non-structural (NS) protein. As a result, 4 out of 23 (17.39%) rats were positive for herpesvirus DNA, but none were positive for WNV RNA and parvovirus DNA. The positive PCR amplicons of herpesvirus DNA were subjected to partial DNA sequencing analysis, 100% identical to Acomys herpesvirus SVMS 226,222 from Betaherpesvirinae, which is highly suggestive of rat cytomegalovirus (RCMV). This study has successfully demonstrated the presence of RCMV from Rattus spp. In the Klang Valley. The RCMV potentially crosses species barriers and establishes infection, raising public health concerns. The non-viraemic state of WNV or parvovirus infection, low sample size, and limited niche distribution emphasise the need for the expansion of this study in the future.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202307080002153ZK.pdf | 716KB | download |