Molecular Identification and Species Richness of Flies (Diptera) and Their Associated Bovidae Hosts at Cattle Farms in Selangor, Malaysia

Pertanika Journal of Tropical Agricultural Science

article

Salmah Yaakop¹  Puteri Amira Amiruddin¹  Muhamad Azmi Mohammed²  Aqilah Sakinah Badrulisham¹  Nadiatur Akmar Zulkifli³  Mohd Noor Hisham Mohd Nadzir⁴ 

[1] Centre for Insect Systematics, Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia;Department of Crop Science, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus;Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia;Department of Biology, Faculty of Science, Universiti Putra Malaysia

关键词: Blood-sucking insect;    COI;    cytb;    DNA barcode;    fly;    host;    livestock;    Malaysia;   

DOI  :  10.47836/pjtas.45.3.05

学科分类：农业科学（综合）

来源: Universiti Putra Malaysia, Universiti Pertanian Malaysia Press

【 摘 要 】

Flies (Diptera) play a significant role in the ecosystem as pollinators and decomposers, and they are also important vermin and disease vectors. Studies on the dipteran species are still lacking in Malaysia; therefore, the dipteran species’ biology, morphology, distribution, and abundance are necessary. The objectives of this study were to identify dipteran species using a molecular approach, determine flies’ Bovidae hosts, and investigate the diversity of the fly’s species at three different cattle farms purposively selected in Selangor, Malaysia. The fly species were identified using cytochrome oxidase subunit I (COI) (Haematopota javana, Tabanus rubidus, Tabanus fontinalis, Iranihindia martellata, Musca domestica, and Chrysomya megacephala), while another six species only up to genus level ( Haematopota sp. 1, Musca sp. 1, Asilus sp., Metopia sp., Anasillomos sp., and Ommatius sp.). In addition, two species of hosts: Bos indicus and Bos taurus, were proven to be the associated host species for the dipteran species based on molecular data of cytochrome b (cytb). However, there were no significant differences between farms in species diversity and richness (F = 1.262, df = 2, p0.05). Interestingly, the two most dominant dipteran genera collected from the cattle farms were Musca and Chrysomya. At the same time, its abundance may have been influenced by the structure of the cattle cage flooring, which serves as a breeding site and food source. These findings contribute to fundamental epidemiological data in developing control strategies for dipteran species and are of great economic and health importance to livestock production in Malaysia.

【 授权许可】

Unknown