EFFECTIVE DIAGNOSTIC TECHNIQUES IN BORRELIA BURGDORFERI INFESTATION IN DOGS By: Maria Larisa IVĂNESCU, Gabriela MARTINESCU and Liviu Dan MIRON Download

Cercetari Economice in Moldova

article

Maria Larisa IVĂNESCU¹  Gabriela MARTINESCU¹  Liviu Dan MIRON¹ 

[1] Iasi University of Life Sciences, Faculty of Veterinary Medicine, Department of Clinics

关键词: Lyme disease;    antibodies;    real-time PCR.;   

DOI  :  10.46909/alse-552059

学科分类：农业科学（综合）

来源: Universitatea de Stiinte Agricole si Medicina Veterinara "Ion Ionescu de la Brad"

【 摘 要 】

Borreliosis or Lyme disease is a disease transmitted by ixodidae ticks during feeding on blood ( Ixodes pacificus and Ixodes scapularis in the USA, Ixodes persulcatus in Asia, Ixodes ricinus in Europe) and is widespread in the entire northern hemisphere. In Romania, the geographic distribution and prevalence of Borrelia burgdorferi sensu lato was 1.4% in 41 counties, with a prevalence between 0.75–18.8%. B. burgdorferi sensu lato. had a prevalence of 3.8%, being found inside ticks in 55 of 183 localities. Successful treatment and full recovery can only be achieved through early diagnosis. The clinical and serologic diagnosis of Lyme disease is particularly difficult because of the phenotypic heterogeneity within and among spirochete species. A case study is presented in this paper: an eight-year-old male Yorkshire terrier dog, which was diagnosed positive for Lyme disease, based on a test which uses a peptide called C6 and which comes from the VlsE protein of B. burgdorferi , used to detect antibodies in dogs. The results demonstrate the reliability of the commercial SNAP 4Dx Plus Test for B. burgdorferi , which uses C6 to differentiate antibodies produced by natural infection from antibodies formed after vaccination. In addition, using real-time PCR, the diagnosis was negative, confirming the results from the literature, according to which the PCR technique is only recommended for research, the positivity percentage being low, especially when the sample is blood (0.1%). We conclude that the tests for the detection of antibodies specific to Lyme disease are recommended and useful.

【 授权许可】

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