Obtaining and characterization of bacterial cellulose synthesized by Komagataeibacter hansenii from alternative sources of nitrogen and carbon

Materia

article

Biassander Camila Tureck¹  Haira Gabriela Hackbarth¹  Eduarda Zeni Neves¹  Michele Cristina Formolo Garcia¹  Giannini Pasiznick Apati¹  Derce de Oliveira Souza Recouvreux²  Ana Paula Testa Pezzin¹  Andrea Lima dos Santos Schneider¹ 

[1] University of Joinville Region;Federal University of Santa Catarina

关键词: Bacterial cellulose;    corn steep liquor;    Prodex Lac®;    nitrogen sources;    carbon sources;   

DOI  :  10.1590/S1517-707620210004.1392

学科分类：工程和技术（综合）

来源: Universidade Federal do Rio de Janeiro * Coordenacao dos Programas de Pos-Graduacao de Engenharia

【 摘 要 】

This work aimed to obtain and characterize bacterial cellulose (BC) membranes obtained by cultivating Komagataeibacter hansenii ATCC 23769 using mannitol, glucose, fructose, lactose, glycerol, inulin, and sucrose as carbon sources, and corn steep liquor and Prodex Lac® as alternative sources of nitrogen. The formation of the BC´s gelatinous membrane was monitored for 12 days under static conditions and a temperature of 30 ºC. After purification, the membranes were dried and characterized by thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The highest BC concentrations were found in the culture medium containing Prodex Lac® as the source of nitrogen. Among sugars, fructose and mannitol presented the best results. TGA analyzes indicate that all membranes have similar thermal behavior. The FTIR results show that the chemically synthesized membranes are equivalent to the structures cited in the literature. The micrographs obtained by SEM showed that the medium might influence BC´s morphology, but in general, all presented nanofibers, an essential feature in the membrane. Thus, the BC membranes synthesized in this study proved that the BC production using low-cost alternative means is feasible. The material obtained meets the expected thermal, physical, and chemical properties.

【 授权许可】

CC BY